Microheater-integrated zinc oxide nanowire microfluidic device for hybridization-based detection of target single-stranded DNA

Detection of cell-free DNA (cfDNA) has an impact on DNA analysis in liquid biopsies. However, current strategies to detect cfDNA have limitations that should be overcome, such as having low sensitivity, and requiring much time and a specialized instrument. Thus, non-invasive and rapid detection tool...

Full description

Saved in:
Bibliographic Details
Published in:Nanotechnology 2021-06, Vol.32 (25), p.255301
Main Authors: Takahashi, Hiromi, Yasui, Takao, Kashida, Hiromu, Makino, Koki, Shinjo, Keiko, Liu, Quanli, Shimada, Taisuke, Rahong, Sakon, Kaji, Noritada, Asanuma, Hiroyuki, Baba, Yoshinobu
Format: Article
Language:English
Subjects:
cell-free DNA
DNA hybridization
microfluidics
microheater
ZnO nanowires
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Detection of cell-free DNA (cfDNA) has an impact on DNA analysis in liquid biopsies. However, current strategies to detect cfDNA have limitations that should be overcome, such as having low sensitivity, and requiring much time and a specialized instrument. Thus, non-invasive and rapid detection tools are needed for disease prevention and early-stage treatment. Here we developed a device having a microheater integrated with zinc oxide nanowires (microheater-ZnO-NWs) to detect target single-stranded DNAs (ssDNAs) based on DNA probe hybridization. We confirmed experimentally that our device realized in-situ annealed DNA probes by which we subsequently detected target ssDNAs. We envision that this device can be utilized for fundamental studies related to nanobiodevice-based DNA detection.
ISSN:0957-4484
1361-6528
DOI:10.1088/1361-6528/abef2c