Radiolabeled HER2-directed exosomes exhibit improved cell targeting and specificity

Here, we established a reliable strategy for generation and characterization of targeted radiolabeled exosomes for the detection of HER2-positive cells quantitatively. Targeted exosomes (T-exos) were radiolabeled by two different radiotracers, [ Tc]Tc-HMPAO or [ In]In-oxine. The labeling efficiency...

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Bibliographic Details
Published in:Nanomedicine (London, England) England), 2021-03, Vol.16 (7), p.553-567
Main Authors: Ghavami, Mahlegha, Vraka, Chrysoula, Hubert, Virginie, Schachner, Helga, Bamminger, Karsten, Hacker, Marcus, Kain, Renate, Moghadam, Mehdi Forouzandeh
Format: Article
Language:English
Subjects:
breast cancer
DARPin
HER2
imaging
radiotracer
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Summary:Here, we established a reliable strategy for generation and characterization of targeted radiolabeled exosomes for the detection of HER2-positive cells quantitatively. Targeted exosomes (T-exos) were radiolabeled by two different radiotracers, [ Tc]Tc-HMPAO or [ In]In-oxine. The labeling efficiency and stability were assessed using exosome exclusive spin columns. HER2-positive and -negative cells were treated with [ In]In-oxine-exosomes after 3 and 24 h. [ In]In-oxine labeling did not change the binding ability and general features of the exosomes. With [ In]In-oxine, 70% labeling efficiency and 78% radiochemical stability over 24 h were achieved. [ In]In-oxine-T-exos showed greater uptake by HER2-positive cells compared with untargeted exosomes. [ In]In-oxine-T-exos could potentially be used as an effective imaging tool for HER2 expression.
ISSN:1743-5889
1748-6963
DOI:10.2217/nnm-2020-0408