Flavisolibacter longurius sp. nov., isolated from soil

• Published in: Archives of microbiology 2021-08, Vol.203 (6), p.2825-2830
• Main Authors: Maeng, Soohyun, Park, Yuna, Lee, Sang Eun, Han, Joo Hyun, Bai, Jaewoo, Kim, Myung Kyum
• Format: Article
• Language: English
• Subjects: Bacteria; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cell Biology; Ecology; Fatty acids; Gene sequencing; Genomes; Gram-negative bacteria; Life Sciences; Lipids; Microbial Ecology; Microbiology; New species; Original Paper; pH effects; Phosphatidylethanolamine; Phylogenetics; Phylogeny; Quinones; rRNA 16S; Soil microorganisms; Soils
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-021-02236-3

A novel Gram-negative bacterial strain BT320 T was isolated from soil collected in Uijeongbu city, Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain BT320 T belong to a distinct lineage within the genus Flavisolibacter (family Chitinophagaceae , order Chitinophagales , class Chitinophagia ). The strain BT320 T was closely related to Flavisolibacter galbus 17J28-26 T (97.6% 16S rRNA gene similarity), Flavisolibacter nicotianae X7X T (96.7%), Flavisolibacter ginsengiterrae Gsoil 492 T (96.2%), and Flavisolibacter ginsengisoli Gsoil 643  T (96.1%). The genome size of strain BT320 T was 5,664,094 bp. Bacterial growth was observed at 10–37 °C (optimum 25 °C) and pH 6.0–8.0 (optimum pH 7.0) on R2A agar. The major cellular fatty acids of strain BT320 T were iso-C 15:0 , summed feature 3 (C 16:1 ω 6 c /C 16:1 ω 7 c ), and summed feature 1 (iso-C 15:1 H/C 13:0 3OH). Its predominant respiratory quinone was MK-7. The major polar lipid of strain BT320 T was identified to be phosphatidylethanolamine (PE). Based on the biochemical, chemotaxonomic, and phylogenetic analysis, strain BT320 T can be suggested as a novel bacterial species within the genus Flavisolibacter and the proposed name is Flavisolibacter longurius. The type strain of Flavisolibacter longurius is BT320 T (= KCTC 72422 T  = NBRC 114375 T ).