Effects of intravenous administration of recombinant Phα1β toxin in a mouse model of fibromyalgia

• Published in: Toxicon (Oxford) 2021-05, Vol.195, p.104-110
• Main Authors: Garcia Mendes, Mariana Peluci, Carvalho dos Santos, Duana, Rezende, Márcio Júnior S., Assis Ferreira, Luana Caroline, Rigo, Flavia Karine, José de Castro Junior, Célio, Gomez, Marcus Vinicius
• Format: Article
• Language: English
• Subjects: Administration, Intravenous; Analgesics - therapeutic use; Animals; Calcium channel blocker; Diclofenac; Disease Models, Animal; Fibromyalgia - drug therapy; Hyperalgesia - drug therapy; Mice; Pregabalin; Recombinant toxin; Reserpine - therapeutic use; Spider Venoms - administration & dosage; Spider Venoms - toxicity; TRPA1 antagonist fibromyalgia
• ISSN: 0041-0101; 1879-3150
• DOI: 10.1016/j.toxicon.2021.03.012

This study investigated the effects of intravenous (iv) administration of recombinant Phα1β toxin, pregabalin, and diclofenac by the intrathecal route using an animal model fibromyalgia (FM). The reserpine administration (0.25 mg/kg s. c) once daily for three consecutive days significantly induced hyperalgesia, immobility time, and sucrose consumption in mice on the 4th day. Reserpine caused hyperalgesia on the mechanical and thermal hyperalgesia on the 4th day was reverted by recombinant Phα1β (0.2 mg/kg iv) and pregabalin (1.25 μmol/site i. t) treatments. In contrast, diclofenac (215 nmol/site i. t) was ineffective. Recombinant Phα1β toxin, pregabalin, and diclofenac did not affect the depressive-like behavioural effect induced by reserpine on mice during the forced swim and sucrose consumption tests. The data confirmed the analgesic effect of the recombinant Phα1β toxin administered intravenously in a fibromyalgia mouse model. 1- Reduced mechanical withdrawal threshold after three consecutive days of reserpine (0,25 mg/kg)”, 2- Intravenous administration of “recombinant Phα1β 0.2 mg/kg “, 3- Two hours after the administration of Phα1β (0.2 mg/kg IV) thresholds increased. [Display omitted] •Recombinant Phα1β iv and pregabalin it reduced reserpine-induced thermal hyperalgesic effect on mice.•Reserpine induced mechanical hyperalgesia in mice was inhibited by recombinant Phα1β and pregabalin.•Reserpine-induced effects on FST and SPT of mice were not inhibited by recombinant Phα1β iv and pregabalin it.•Diclofenac had no effect on the reserpine-induced mechanical and thermal hyperalgesia of mice.