Differential miRNA profiles in South African cassava mosaic virus-infected cassava landraces reveal clues to susceptibility and tolerance to cassava mosaic disease

•Cassava mosaic disease susceptible T200 and tolerant TME3 landraces display distinct miRNA profiles.•MicroRNA expression patterns in response to South African cassava mosaic virus are infection-stage specific.•Early RNA silencing response in TME3 prior to symptom development is key to tolerance/rec...

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Bibliographic Details
Published in:Virus research 2021-10, Vol.303, p.198400-198400, Article 198400
Main Authors: Bizabani, Christine, Rogans, Sarah Jane, Rey, Marie Emma Chrissie
Format: Article
Language:English
Subjects:
Begomovirus - genetics
Cassava
Disease Susceptibility
Indoleacetic Acids
Manihot - genetics
MicroRNAs - genetics
miRNAs
Plant Diseases
Posttranscriptional gene silencing (PTGS)
South African cassava mosaic virus
Susceptibility
Tolerance
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Summary:•Cassava mosaic disease susceptible T200 and tolerant TME3 landraces display distinct miRNA profiles.•MicroRNA expression patterns in response to South African cassava mosaic virus are infection-stage specific.•Early RNA silencing response in TME3 prior to symptom development is key to tolerance/recovery.•A role for auxin signalling in recovery of induced symptoms in TME3 is proposed. Specific miRNA families are involved in susceptibility or antiviral immunity in plants. Manihot esculenta Crantz (cassava) is a perennial plant that is an important food security crop in sub-Saharan Africa. Cassava is susceptible to several begomoviruses that cause cassava mosaic disease (CMD). In this study, we investigated the leaf miRNAome response in a tolerant (TME3) and susceptible (T200) cassava landrace challenged with South African cassava mosaic virus. RNAseq was performed on leaf samples at 12, 32 and 67 days post infection (dpi), representing early, symptomatic and late persistent stages of CMD infection. Significantly, distinct profiles of conserved miRNA family expression between the T200 and TME3 landraces at the three infection stages were observed. Notably at 12 days post SACMV infection, TME3 exhibited significant downregulation (log2fold
ISSN:0168-1702
1872-7492
DOI:10.1016/j.virusres.2021.198400