Peptidomic changes of saliva after non‐surgical treatment of stage I/II generalized periodontitis

Objective To explore the changes of peptidome profiles of saliva, serum, and gingival crevicular fluid (GCF) before and after non‐surgical periodontal treatment in patients with generalized periodontitis (stage I/II). Subjects and methods Saliva, serum, and GCF samples were collected from 17 patient...

Full description

Saved in:
Bibliographic Details
Published in:Oral diseases 2022-09, Vol.28 (6), p.1640-1651
Main Authors: Yuan, Chao, Ma, Zhangke, Tong, Peiyuan, Yu, Shunlan, Li, Yi, Gallagher, Jennifer Elizabeth, Sun, Xiangyu, Zheng, Shuguo
Format: Article
Language:English
Subjects:
chronic periodontitis
Gum disease
Haptoglobin
Immunoglobulins
Ions
Liquid chromatography
Mass spectrometry
Mass spectroscopy
non‐surgical treatment
Patients
Peptides
Periodontal diseases
Periodontitis
Saliva
Scientific imaging
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objective To explore the changes of peptidome profiles of saliva, serum, and gingival crevicular fluid (GCF) before and after non‐surgical periodontal treatment in patients with generalized periodontitis (stage I/II). Subjects and methods Saliva, serum, and GCF samples were collected from 17 patients at baseline (T0), one week after ultrasonic supragingival scaling (T1) and eight weeks after subgingival scaling and root planning (T2). Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) was carried out to detect changes in peptidomic profiles. Then, nano‐liquid chromatography‐electrospray ionization‐tandem mass spectrometry (nano‐LC/ESI‐MS/MS) was performed to identify potential peptide biomarkers. Results Most of the peptides from the patients exhibited a decreasing trend from the time point of pretreatment to that of post‐treatment. Cluster analysis and scatter plots using these peptides indicated that salivary peptidome has an acceptable capability of reflecting the status of stage I/II generalized periodontitis. Seven of these peptides were successfully identified as α‐1‐antitrypsin, immunoglobulin κ variable 4‐1, haptoglobin, and immunoglobulin heavy constant γ2. Conclusions Certain peptides in saliva, serum, and GCF were down‐regulated after non‐surgical periodontal treatment, demonstrating the application prospects of saliva in monitoring and surveillance of periodontal diseases in both clinical settings and communities.
ISSN:1354-523X
1601-0825
DOI:10.1111/odi.13838