Rational Design of a Dual-Layered Metal–Organic Framework Nanostructure for Enhancing the Cell Imaging of Molecular Beacons

As the most popular nucleic acid probe, molecular beacons (MBs) can selectively light up endogenous RNA targets without specific treatment. However, the poor cell permeability and unsatisfied intracellular stability of MBs significantly restricted their detection performance. Herein, we report the e...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2021-04, Vol.93 (13), p.5437-5441
Main Authors: Gao, Peng, Lou, Ruxin, Liu, Xiaohan, Cui, Bingjie, Pan, Wei, Li, Na, Tang, Bo
Format: Article
Language:English
Subjects:
Beacons
Cell permeability
Chemistry
Imaging
Intracellular
MDR1 protein
Metal-organic frameworks
mRNA
Multidrug resistance
Nanoparticles
Nanostructure
Nucleic acids
P-Glycoprotein
Surface stability
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Summary:As the most popular nucleic acid probe, molecular beacons (MBs) can selectively light up endogenous RNA targets without specific treatment. However, the poor cell permeability and unsatisfied intracellular stability of MBs significantly restricted their detection performance. Herein, we report the encapsulation of MB within a dual-layered metal–organic framework nanostructure UiO66-ZIF8 for enhanced cell imaging. UiO66-NH2 nanoparticles were synthesized as the template for MB loading, and the ZIF-8 shell was further coated on the surface of UiO66-MB to ensure its stability and lysosomal escape effect. Taking multidrug-resistant (MDR1) mRNA as a model target, MBs loaded within UiO66-ZIF8 showed an improved lysosomal escape effect compared with MB absorbed on UiO66-NH2. Therefore, efficient and accurate intracellular MDR1 mRNA imaging was realized with UiO66-MB-ZIF8. This work presented a new method for the rational regulation of the intracellular fate of MOF-based nanoprobes and will facilitate the further development of hierarchical MOF nanoprobes for analytical applications.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.0c05060