Antithrombotic and anticoagulant effects of a novel protein isolated from the venom of the Deinagkistrodon acutus snake

• Published in: Biomedicine & pharmacotherapy 2021-06, Vol.138, p.111527-111527, Article 111527
• Main Authors: Huang, Jin, Song, Wei, Hua, Haibing, Yin, Xiaojian, Huang, Fang, Alolga, Raphael N.
• Format: Article
• Language: English
• Subjects: Antithrombotic; Deinagkistrodon acutus venom; Fibrinogen; Metabolomics; SLPC
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/j.biopha.2021.111527

The venom of the Deinagkistrodon acutus snake is composed of numerous bioactive proteins and peptides. In this study, we report the antithrombotic and anticoagulant activities of one of such proteins, herein known as SLPC. This novel protein was isolated and purified via multi-gel chromatography. Its amino acid sequence, structure and function were then determined. This protein was found to exhibit defibration, anticoagulation and general antithrombotic effects based on the results of both in vitro and in vivo studies. Based on same studies, it was found to cleave the α, β, γ chains of fibrinogen and generally improved antiplatelet aggregation and blood rheology. A metabolomic insight of the antithrombotic effects of SLPC was found to be mainly linked to perturbations in the synthesis of unsaturated fatty acids, glycerophospholipid metabolism, arachidonic acid metabolism and other metabolic pathways. In summary, the novel protein SLPC, elicits its antithrombotic effects via degradation of fibrinogen and regulation of various thrombogenic factors in multiple metabolic pathways. [Display omitted] •A novel protein named SLPC was isolated from the venom of Deinagkistrodon acutus.•SLPC possesses defibration, anticoagulation and general antithrombotic effects.•SLPC was found to cleave the α, β, γ chains of fibrinogen.•Metabolomics showed its effects are linked to perturbed lipids metabolism.