Near-Infrared Mitochondria-Targetable Fluorescent Probe for High-Contrast Bioimaging of H2S

To elucidate the complex role of biological H2S and study the mitochondrial damage and some related diseases, effective methods for visualization of H2S in mitochondria and in vivo are urgently needed. In this contribution, a novel near-infrared mitochondria-targetable fluorescence probe MI-H 2 S fo...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 2021-04, Vol.93 (14), p.5700-5708
Main Authors: Gong, Shengyi, Zheng, Zhoupeng, Guan, Xiaogang, Feng, Shumin, Feng, Guoqiang
Format: Article
Language:English
Subjects:
Aqueous solutions
Chemistry
Fluorescence
Fluorescent indicators
Hydrogen sulfide
I.R. radiation
In vivo methods and tests
Inflammation
Medical imaging
Mitochondria
Visualization
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To elucidate the complex role of biological H2S and study the mitochondrial damage and some related diseases, effective methods for visualization of H2S in mitochondria and in vivo are urgently needed. In this contribution, a novel near-infrared mitochondria-targetable fluorescence probe MI-H 2 S for H2S detection was developed. MI-H 2 S shows rapid detection ability for H2S in pure aqueous solution and outputs a highly selective and sensitive fluorescence-on signal at 663 nm with a large Stokes shift of 141 nm. Bioimaging experiments revealed that the probe has good mitochondrial-targeting ability and high-contrast imaging ability for detecting H2S in living systems. The probe also showed great potential in the detection of H2S during inflammation. All of the results demonstrate that MI-H 2 S can be applied as an effective probe for the visualization and study of H2S in mitochondria and in vivo.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.0c04639