Therapeutic effect of an anti-tuberculosis agent, isoniazid, and its nano-isoform in ulcerative colitis

• Published in: International immunopharmacology 2021-07, Vol.96, p.107577-107577, Article 107577
• Main Authors: Yaghoubi, Atieh, Davoodi, Javid, Asgharzadeh, Fereshteh, Rezaie, Sajad, Nazari, Elnaz, Khazaei, Majid, Soleimanpour, Saman
• Format: Article
• Language: English
• Subjects: Animals; Anti-inflammatory agents; Anti-Inflammatory Agents - therapeutic use; Antitubercular Agents - therapeutic use; Colitis, Ulcerative - drug therapy; Colon; Dextran Sulfate; Diarrhea; Disease Models, Animal; Drug Combinations; Histology; Humans; Inflammation; Inflammatory bowel disease; Inflammatory bowel diseases; INH; Interleukin 1; Intestinal Mucosa - pathology; Isoniazid; Isoniazid - therapeutic use; Male; Mice; Mice, Inbred C57BL; Mucosa; Nanoparticle; Nanoparticles; Nanoparticles - therapeutic use; NF-κB protein; Signs and symptoms; Spleen; Sulfasalazine; Sulfasalazine - therapeutic use; Transcription factors; Tuberculosis; Ulcerative colitis; Weight
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2021.107577

•Combination therapy of INH with sulfasalazine can significantly reduce the symptom of ulcerative colitis.•INH and Sulfasalazine-loaded nanoparticles can significantly decrease the symptom of ulcerative colitis.•Combination therapy of INH with sulfasalazine can improved disease activity index.•INH and Sulfasalazine-loaded NPs in comparison to the combination therapy enhances the therapeutic effect of the drug. Isoniazid (INH) is well known as a first-line anti-tuberculosis, while some studies demonstrate that it has anti-inflammatory activity via a different mechanism such as inhibitionthe production of IL-1, ROS, activation of PPARγ expression, inhibition of the transcriptional regulatory activity of NF-κB and AP-1. The aim of this study, investigate the anti-inflammatory effect of INH and INH combined with Sulfasalazine-loaded nanoparticles (NPs) in the ulcerative colitis mouse model. To investigate the anti-inflammatory effect of INH and NPs in the ulcerative colitis mice model, we evaluated the effect of INH clinical symptoms and colonic mucosal histology in colitis. The present study demonstrates that combination therapy of INH with sulfasalazine as well as NPs reduces the symptom of ulcerative colitis and improved disease activity index include body lose weight, diarrhea, rectal bleeding, colonic length, spleen weight, and colon histopathological score in DSS-induced colitis mice model. Our results suggest that the nanoforms of INH with sulfasalazine enhances the therapeutic effect of the drugs in the treatment of ulcerative colitis.