Assembly of Model Membrane Nanodiscs for Native Mass Spectrometry

Native mass spectrometry (MS) with nanodiscs is a promising technique for characterizing membrane protein and peptide interactions in lipid bilayers. However, prior studies have used nanodiscs made of only one or two lipids, which lack the complexity of a natural lipid bilayer. To better model speci...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2021-04, Vol.93 (14), p.5972-5979
Main Authors: Kostelic, Marius M, Zak, Ciara K, Jayasekera, Hiruni S, Marty, Michael T
Format: Article
Language:English
Subjects:
Analytical chemistry
Antiinfectives and antibacterials
Antimicrobial peptides
Assembly
Biological membranes
Chemistry
Complexity
Fluidity
Lipid bilayers
Lipids
Mass spectrometry
Mass spectroscopy
Membrane proteins
Membranes
Mitochondria
Peptides
Phospholipids
Proteins
Scientific imaging
Spectroscopy
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Summary:Native mass spectrometry (MS) with nanodiscs is a promising technique for characterizing membrane protein and peptide interactions in lipid bilayers. However, prior studies have used nanodiscs made of only one or two lipids, which lack the complexity of a natural lipid bilayer. To better model specific biological membranes, we developed model mammalian, bacterial, and mitochondrial nanodiscs with up to four different phospholipids. Careful selection of lipids with similar masses that balance the fluidity and curvature enabled these complex nanodiscs to be assembled and resolved with native MS. We then applied this approach to characterize the specificity and incorporation of LL-37, a human antimicrobial peptide, in single-lipid nanodiscs versus model bacterial nanodiscs. Overall, development of these model membrane nanodiscs reveals new insights into the assembly of complex nanodiscs and provides a useful toolkit for studying membrane protein, peptide, and lipid interactions in model biological membranes.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.1c00735