Nanohybrid composed of graphene oxide functionalized with sodium hyaluronate accelerates bone healing in the tibia of rats

• Published in: Materials Science & Engineering C 2021-04, Vol.123, p.111961-111961, Article 111961
• Main Authors: Dantas, Paulo César de Lacerda, Martins-Júnior, Paulo Antônio, Coutinho, Danielle Carvalho Oliveira, Andrade, Vanessa Barbosa, Valverde, Thalita Marcolan, Ávila, Erick de Souza, Almeida, Tatiane Cristine Silva, Queiroz-Junior, Celso Martins, Sá, Marcos Augusto, Góes, Alfredo Miranda, Ladeira, Luiz Orlando, Ferreira, Anderson José, Marques, Leandro Silva
• Format: Article
• Language: English
• Subjects: Animals; Biomedical materials; Blood coagulation; Bone growth; Bone healing; Bone repair; Bone tissue engineering; Cell viability; Covalent bonds; Defects; Evaluation; Fourier transforms; Graphene; Graphite - pharmacology; Growth factors; Hyaluronic Acid - pharmacology; In vivo methods and tests; Infrared analysis; Infrared spectroscopy; Materials science; Nanomaterial; Rats; Rats, Wistar; Regeneration; Scanning electron microscopy; Scientific imaging; Sodium; Sodium hyaluronate; Spectrometry; Synthesis; Thermogravimetric analysis; Thermogravimetry; Tibia; Tissue engineering; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A; X-ray diffraction
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2021.111961

This study synthesized and characterized a nanohybrid composed of graphene oxide (GO) functionalized with sodium hyaluronate (HY) (GO-HY), evaluated its effect in vitro and determined its osteogenic potential in vivo. The synthesized nanohybrid was analyzed by Scanning electron microscopy (SEM), Raman spectrometry, Thermogravimetry, Fourier-transform infrared (FTIR) spectroscopy and X-ray diffraction. MC3T3-E1 cell viability was assessed by MTT assay in 48 and 72 h. Bone defects were created in tibia of 40 Wistar rats and filled with blood clot (control), 1% HY, GO (50, 100 and 200 μg/mL) and the nanohybrid (50, 100 and 200 μg/mL). After 7 and 14 days, histomorphometric analysis was carried out to assess osteogenic potential of the nanohybrid. Immunohistochemical analysis evaluated the expression of vascular endothelial growth factor (VEGF) in bone defects. Thermogravimetric analysis, Raman and FTIR spectrometry confirmed the functionalization of GO with HY by covalent bonds. Five μg/mL concentrations of the nanohybrid did not alter the viability of the MC3T3-E1 cells. Histomorphometric analysis demonstrated that the nanohybrid at 100 μg/mL significantly accelerated the bone repair in tibia of rats when compared to controls (p