Herbal hydrogel‐based encapsulated Enterococcus faecium ABRIINW.N7 improves the resistance of red hybrid tilapia against Streptococcus iniae

• Published in: Journal of applied microbiology 2021-11, Vol.131 (5), p.2516-2527
• Main Authors: Kahieshesfandiari, M., Nami, Y., Lornezhad, G., Kiani, A., Javanmard, A., Jaymand, M., Haghshenas, B.
• Format: Article
• Language: English
• Subjects: Alginates; Alginic acid; basil seed; Biological activity; Cell survival; Cell viability; Economic impact; Encapsulation; Enterococcus faecium; Fenugreek; Histopathology; Hydrogels; Microbial activity; Microorganisms; Mortality; probiotic Enterococcus faecium; Probiotics; Process parameters; red hybrid tilapia; Streptococcus iniae; Survival; Tilapia; Trigonella foenum-graecum
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/jam.15098

Aims The streptococcal disease has been associated with serious mortality and significant global economic loss in the tilapia farming industry. The overall goal of this work was to test herbal hydrogels based on encapsulated Enterococcus faecium ABRIINW.N7 for potential probiotic anti‐microbial activity against Streptococcus iniae in red hybrid tilapia. Methods and Results Abnormal behaviour, clinical signs, postinjection survival and histopathology (kidney, liver, eye and brain) were measured. Cumulative mortality of CON+, free cells, ALG and treatments (F1‐F7) was 30, 24, 22, 19, 17, 17, 16, 14, 14 and 12 out of 30 fish and the survival rates for E. faecium ABRIINW.N7 microencapsulated in an alginate‐BS blend with 0·5, 1, 1·5, 2, 2·5 and 3% fenugreek were 43, 43, 47, 53, 53 and 60%, respectively. After the incorporation of fenugreek with the alginate‐BS blend, there was an 8–21% increase in probiotic cell viability. Furthermore, the survival rate for the alginate‐BS blend with 2·5 and 3% fenugreek (F6 and F7) was significantly (P ≤ 0·05) higher than other blends. The highest encapsulation efficiency, viability in gastrointestinal conditions and during storage time and excellent antipathogenicity against S. iniae were observed in alginate‐BS +3% fenugreek formulation (F7). Conclusions It is recommended that probiotic strains like E. faecium ABRIINW.N7 in combination with local herbal gums, such as BS and fenugreek plus alginate, can be used as a suitable scaffold and an ideal matrix for the encapsulation of probiotics. Significance and Impact of the Study This study proposes models connecting process parameters, matrix structure and functionality.