In vivo local transcranial static magnetic field stimulation alters motor behavior in normal rats

• Published in: Neuroscience letters 2021-05, Vol.753, p.135864-135864, Article 135864
• Main Authors: Takamatsu, Yasuyuki, Inoue, Takahiro, Okamura, Misato, Ikegami, Ryo, Maejima, Hiroshi
• Format: Article
• Language: English
• Subjects: Behavior; Cortex; Inhibition; Neuromodulation; Static magnetic fields
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/j.neulet.2021.135864

•Transcranial static magnetic field stimulation (tSMS) in rats is feasible in awake and free moving conditions.•tSMS does not affect the activity level and symmetry of forelimb use.•tSMS over the primary motor cortex has inhibitory neuromodulatory effects on motor behavior. Transcranial static magnetic field stimulation (tSMS) has inhibitory neuromodulatory effects on the human brain. Most of the studies on static magnetic fields have been performed in vitro. To further understand the biological mechanisms of tSMS, we investigated the effects of in vivo tSMS on motor behavior in normal awake rats. The skull of a male Wistar rat was exposed and a polyethylene tube was attached to the skull using dental cement at the center of the motor cortex (n = 7) or the other cortex (n = 6). By attaching a cylindrical NdFeB neodymium magnet into the tube, in vivo tSMS (REAL) was performed. For SHAM, we applied a similar size non-magnetic stainless-steel cylinder. All rats received twice each SHAM and REAL stimulation every two days using a crossover design, and motor function was measured during the stimulation. Activity level and asymmetry of forelimb use were not affected, but less accurate movements in the horizontal ladder test were found in REAL stimulation of the motor cortex. This study shows that in vivo tSMS has inhibitory neuromodulatory effects on motor behavior depending on the stimulated region on the rat cortex.