Precisely Encoded Barcodes through the Structure‐Fluorescence Combinational Strategy: A Flexible, Robust, and Versatile Multiplexed Biodetection Platform with Ultrahigh Encoding Capacities

• Published in: Small (Weinheim an der Bergstrasse, Germany) Germany), 2021-05, Vol.17 (19), p.e2100315-n/a
• Main Authors: Wang, Yao, Chen, Cang, He, Jing, Cao, Yimei, Fang, Xiaoxia, Chi, Xiaomei, Yi, Jingwei, Wu, Jiancong, Guo, Qingsheng, Masoomi, Hajar, Wu, Chongzhao, Ye, Jian, Gu, Hongchen, Xu, Hong
• Format: Article
• Language: English
• Subjects: Bar codes; barcodes; Enlargement; Flow cytometry; Fluorescein; Fluorescence; mesoporous microbeads; multiplexed detection; Multiplexing; Nanoparticles; Nanotechnology; Quantum dots; Robustness; structure‐fluorescence; ultrahigh encoding capacities
• ISSN: 1613-6810; 1613-6829
• DOI: 10.1002/smll.202100315

With the rapid development of suspension array technology, microbeads‐based barcodes as the core element with sufficient encoding capacity are urgently required for high‐throughput multiplexed detection. Here, a novel structure‐fluorescence combinational encoding strategy is proposed for the first time to establish a barcode library with ultrahigh encoding capacities. Based on the never revealed transformability of the structural parameters (e.g., porosity and matrix component) of mesoporous microbeads into scattering signals in flow cytometry, the enlargement of codes number has been successfully realized in combination with two other fluorescent elements of fluorescein isothiocyanate isomer I (FITC) and quantum dots (QDs). The barcodes are constructed with precise architectures including FITC encapsulated within mesopores and magnetic nanoparticles as well as QDs immobilized on the outer surface to achieve the ultrahigh encoding level of 300 accompanied with superparamagnetism. To the best of knowledge, it is the highest record of single excitation laser‐based encoding capacity up to now. Moreover, a ten‐plexed tumor markers bioassay based on the tailored‐designed barcodes has been evaluated to confirm their feasibility and effectiveness, and the results indicate that the barcodes platform is a promising and robust tool for practical multiplexed biodetection. A microbeads‐based barcode library with ultrahigh encoding capacities has been successfully established by utilizing a novel structure‐fluorescence combinational encoding strategy. Based on the tailored‐designed barcodes with precise architectures, the feasibility and effectiveness for multiplexed bioassay are demonstrated, indicating the promising potential of the barcodes platform as a robust weapon in practical applications.