Study of the interaction mechanism between hydrophilic thiol capped gold nanoparticles and melamine in aqueous medium

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2021-07, Vol.203, p.111727-111727, Article 111727
• Main Authors: Cerra, Sara, Salamone, Tommaso A., Sciubba, Fabio, Marsotto, Martina, Battocchio, Chiara, Nappini, Silvia, Scaramuzzo, Francesca A., Li Voti, Roberto, Sibilia, Concita, Matassa, Roberto, Beltrán, Ana Maria, Familiari, Giuseppe, Fratoddi, Ilaria
• Format: Article
• Language: English
• Subjects: 3-mercapto-1-propanesulfonate; Aggregation mechanism; Electrostatic interactions; Hydrophilic gold nanoparticles; Melamine
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2021.111727

[Display omitted] •Hydrophilic gold nanoparticles for melamine detection were prepared.•The melamine was successfully detected in the range 2.5−500 ppm.•The sensing method based on the melamine-induced aggregation of AuNPs.•The recognition process is mediated by electrostatic interactions. In the last years, intense efforts have been made in order to obtain colloidal-based systems capable of pointing out the presence of melamine in food samples. In this work, we reported about the recognition of melamine in aqueous solution, using gold nanoparticles stabilized with 3-mercapto-1-propanesulfonate (AuNPs-3MPS), with the aim of deepening how the recognition process works. AuNPs were synthesized using a wet chemical reduction method. The synthesized AuNPs-3MPS probe was fully characterized, before and after the recognition process, by both physicochemical (UV–vis, FT-IR, 1H-NMR, DLS and ζ-potential) and morphostructural techniques (AFM, HR-TEM). The chemical and electronic structure was also investigated by SR-XPS. The sensing method is based on the melamine-induced aggregation of AuNPs; the presence of melamine was successfully detected in the range of 2.5−500 ppm. The results achieved also demonstrate that negatively charged AuNPs-3MPS are potentially useful for determining melamine contents in aqueous solution. SR-XPS measurements allowed to understand interaction mechanism between the probe and the analyte. The presence of sulfonate groups allows a mutual interaction mediated by electrostatic bonds between nanoparticles surface thiols and positively charged amino groups of melamine molecules.