Antioxidant and functional properties of cowhide collagen peptides

In the present study, antioxidant activities and functional properties of cowhide collagen antioxidant peptides (CCAPs) with different molecular weight (MW) were investigated. The optimum preparation conditions of CCAPs were hydrolysis time of 1.53 hr, temperature of 54.9 °C, pH 7.38, and neutral en...

Full description

Saved in:
Bibliographic Details
Published in:Journal of food science 2021-05, Vol.86 (5), p.1802-1818
Main Authors: Xie, Zhike, Wang, Xuguang, Yu, Shuyan, He, Ming, Yu, Shaoxuan, Xiao, Haifang, Song, Yuanda
Format: Article
Language:English
Subjects:
antioxidant activity
Antioxidants
Collagen
collagen peptide
Foaming
Free radicals
Freeze drying
functional property
Hydroxyl radicals
Hygroscopicity
Molecular weight
Peptides
Response surface methodology
Scavenging
Sulfonic acid
Trypsin
Ultrafiltration
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In the present study, antioxidant activities and functional properties of cowhide collagen antioxidant peptides (CCAPs) with different molecular weight (MW) were investigated. The optimum preparation conditions of CCAPs were hydrolysis time of 1.53 hr, temperature of 54.9 °C, pH 7.38, and neutral enzyme to trypsin ratio of 0.048 g: 0.016 g according to single factor test and response surface methodology (RSM). Three fractions (CCAP‐I, CCAP‐II, and CCAP‐III) were obtained by ultrafiltration and lyophilization. Antioxidant activities revealed that CCAP‐III had high reducing power activity (0.323 ± 0.035) and scavenging effect on 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) radicals (64.30 ± 5.99%), 2,2‐azino‐bis‐(3‐ethylbenzothiazoline)‐6‐sulfonic acid (ABTS) radicals (75.25 ± 3.14%), and hydroxyl radicals (68.26 ± 6.74%) compared to the other fractions. In addition, LC‐MS/MS analysis showed that Ala‐Gly‐Glu‐Arg, Gly‐Ile‐Ala‐Gly‐Glu‐Arg, Gly‐Pro‐Ala‐Gly‐Pro‐Ala‐Gly‐Pro‐Arg, Gly‐Val‐Val‐Gly‐Pro‐Glu‐Gly‐Ala‐Arg and Gly‐Phe‐Ser‐Gly‐Leu‐Asp‐Gly‐Ala‐Lys were the major peptides of CCAP‐III. CCAP‐III showed good hygroscopicity (HYG), water holding capacity (WHC), and oil holding capacity (OHC) when compared with CCAP‐I and CCAP‐II. However, CCAP‐II has great emulsifying properties, and CCAP‐I has excellent foaming properties. Therefore, CCAPs can be used as a promising source of functional peptides with antioxidant properties. Practical Application This study demonstrated the peptides of cowhide collagen has superior antioxidant and functional properties. This study provided a scientific basis for the preparation of antioxidant peptides from cowhide collagen.
ISSN:0022-1147
1750-3841
DOI:10.1111/1750-3841.15666