Bioluminescence reporter for monitoring G2-phase cell cycle arrest in vivo

The bioengineered luciferase reporter has been widely used for monitoring of a variety of molecular events in living cells because of their ability to provide highly sensitive quantitation with broad linearity. In the present study, we made a cyclin A2-luciferase (CYCA-Luc) fusion protein and examin...

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Bibliographic Details
Published in:Pakistan journal of pharmaceutical sciences 2020-09, Vol.33 (5), p.2083-2088
Main Authors: Yu, Jian-Bo, Jing, Ya-Jie, Jin, Zai-Shun, Li, Qi, Meng, Xiang-Yu, Chen, Zhi-Hong
Format: Article
Language:English
Subjects:
Cancer screening
Cell cycle
Health aspects
Journalists
Lithium compounds
Luciferase
Online Access:Get full text
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Summary:The bioengineered luciferase reporter has been widely used for monitoring of a variety of molecular events in living cells because of their ability to provide highly sensitive quantitation with broad linearity. In the present study, we made a cyclin A2-luciferase (CYCA-Luc) fusion protein and examined the utility of this optical reporter for monitoring G2-phase cell cycle arrest in living animals. In vitro luciferase assay and in vivo bioluminescence imaging assay showed that the lithium chloride (LiCl), G2-phase-specific drug, induced G2-phase arrest of cell cycle and increased the activity of this reporter under in vitro or in vivo conditions, and this reporter can also be potentially used in high-throughput screening efforts aimed at discovering novel anti-cancer drugs that will cause cell cycle arrest at the G2-phase in cultivated cell lines and animal models.
ISSN:1011-601X
DOI:10.36721/PJPS.2020.33.5.REG.2083-2088.1