A novel inflammatory signaling pathway in patients with slow coronary flow: NF-κB/IL-1β/nitric oxide

• Published in: Cytokine (Philadelphia, Pa.) Pa.), 2021-07, Vol.143, p.155511-155511, Article 155511
• Main Authors: Roshanravan, Neda, Shabestari, Alireza Namazi, Alamdari, Naimeh Mesri, Ostadrahimi, Alireza, Separham, Ahmad, Parvizi, Rezayat, Jafarabadi, Mohammad Asghari, Ghodrat, Mahshid, Akbarzadeh, Moloud, Naemi, Mohammad, Ghazi, Mahdiyeh Khabbaz Koche, Hadi, Amir, Ghaffari, Samad
• Format: Article
• Language: English
• Subjects: Antioxidants - metabolism; Confidence Intervals; Coronary Circulation - physiology; Cytokines - genetics; Cytokines - metabolism; Female; Glutathione Peroxidase - metabolism; Humans; IL-1β; Inflammation; Inflammation - metabolism; Interleukin-1beta - metabolism; Male; Metabolic syndrome; Middle Aged; NF-kappa B - metabolism; NF-κB; Nitric oxide; Nitric Oxide - metabolism; Odds Ratio; RNA, Messenger - genetics; RNA, Messenger - metabolism; Signal Transduction; Slow coronary flow
• ISSN: 1043-4666; 1096-0023
• DOI: 10.1016/j.cyto.2021.155511

The slow coronary flow (SCF) was identified as delayed opacification of epicardial coronary arteries in the absence of stenotic lesion. Metabolic syndrome (MetS), oxidative stress, and inflammation may be possible known insulting factors for the pathogenesis of SCF. This investigation aimed to assess the relationship between some inflammatory markers, oxidative stress parameters and MetS components with SCF phenomenon. A total of 35 patients with SCF and 35 subjects with normal coronary flow (NCF) were included in the study. We assessed some inflammatory markers (IL-1β, IL-18, TNF-α, and NF-κB mRNA expression in peripheral blood mononuclear cells (PBMCs)). Moreover, blood samples of the participants were tested for total antioxidant capacity (TAC), glutathione peroxidase (GPX) and nitric oxide (NO) levels using enzyme-linked immunosorbent assay (ELISA). Diagnosis of MetS was based on the National Cholesterol Education Program’s Adult Treatment Panel III report (ATPIII) criteria, 2005. Diagnostic criteria for coronary flow rates of all subjects were documented by thrombolysis in myocardial infarction (TIMI) frame count method. SCF patients had significantly higher prevalence of MetS (46%, p = 0.048).We found that the level of TAC was significantly higher in the NCF group (p = 0.006). Furthermore, the NO concentration was significantly lower in SCF groups (p = 0.001). A significant incremental difference was detected in IL-1β (fold change 2.82 ± 0.31, p