Bariatric surgery induces morphological changes in the extensor digitorum longus muscle in the offspring of obese rats

• Published in: Tissue & cell 2021-10, Vol.72, p.101537-101537, Article 101537
• Main Authors: Kuhn, C., Covatti, C., Ribeiro, L.F.C., Balbo, S.L., Torrejais, M.M.
• Format: Article
• Language: English
• Subjects: Animals; Bariatric Surgery - adverse effects; Connective Tissue - pathology; Diet; Female; Fetal development; Gastric bypass; Gastrointestinal surgery; Light microscopy; Metabolism; Microscopy; Morphology; Morphometry; Muscle, Skeletal - pathology; Muscle, Skeletal - ultrastructure; Muscles; Neuromuscular junction; Neuromuscular Junction - pathology; Neuromuscular junctions; Obesity; Obesity - surgery; OCT; Offspring; Optical Cohernece Tomography; Optical microscopy; Pregnancy; Rats; Rats, Wistar; Roux-en-Y; Skeletal muscle; Skeletal muscle fibers; Surgery; Transmission electron microscopy; Weaning
• ISSN: 0040-8166; 1532-3072
• DOI: 10.1016/j.tice.2021.101537

[Display omitted] •Skeletal muscle morphological changes in skeletal muscle of offspring following RYGB surgery.•The offspring that were born form the RYGB had a lower weight and adiposity.•Reduction in the fibers area and a nucleus/fiber ratio, as well as a reduction in the area of the NMJs.•The RYGB surgery in mothers produced morphological changes in the skeletal striated muscles of the offspring. The effects of the maternal nutritional environment on the growth and metabolism of the offspring, and its impacts on health in adult life are defined as metabolic programming. Thus, the objective of this study was to evaluate the effects of Roux-en-Y gastric bypass (RYGB) on the morphology of muscle fiber and neuromuscular junction (NMJ) of the offspring of rats submitted to RYGB. Three-week-old Wistar rats were separated into two groups: 1) CAF SHAM which received a cafeteria diet and was submitted to a sham operation and 2) CAF RYGB, which received a cafeteria diet and was submitted to RYGB. The first generation (F1) offspring (male) was named according to the treatment of mothers as CAF SHAM-F1 and CAF RYGB-F1 and received a standard diet after weaning. At 17 weeks, the animals were euthanized, and the extensor digitorum longus muscle (EDL) was collected and processed in light microscopy and transmission electron microscopy for morphological and morphometric analysis. The CAF RYGB-F1 group showed a reduction in the weight of the EDL muscle and also a reduction in the area of type I, IIa and IIb fibers and a nucleus/fiber ratio. This same group also showed an increase in the capillary density and myofibrillar disorganization and in the Z-line, as well as a reduction in the area of the NMJs. The RYGB surgery in mothers produced morphological changes in the skeletal striated muscles of the offspring.