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Effect of Akkermansia muciniphila, Faecalibacterium prausnitzii, and Their Extracellular Vesicles on the Serotonin System in Intestinal Epithelial Cells
The gastrointestinal (GI) tract is an essential reservoir of serotonin or 5-hydroxytryptamine (5-HT), which possesses a set of bacterial species communities. Intestinal microbiota has the ability to modulate the host’s serotonin system. In this regard, we evaluated the effect of Akkermansia muciniph...
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Published in: | Probiotics and antimicrobial proteins 2021-12, Vol.13 (6), p.1546-1556 |
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creator | Yaghoubfar, Rezvan Behrouzi, Ava Zare Banadkoki, Ehsan Ashrafian, Fatemeh Lari, Arezou Vaziri, Farzam Nojoumi, Seyed Ali Fateh, Abolfazl Khatami, Shohreh Siadat, Seyed Davar |
description | The gastrointestinal (GI) tract is an essential reservoir of serotonin or 5-hydroxytryptamine (5-HT), which possesses a set of bacterial species communities. Intestinal microbiota has the ability to modulate the host’s serotonin system. In this regard, we evaluated the effect of
Akkermansia muciniphila
and
Faecalibacterium prausnitzii
along with their extracellular vesicles (EVs) on serotonin system-related genes in human epithelial colorectal adenocarcinoma (Caco-2) cells. The differentiated Caco-2 cells were treated with
A. muciniphila
and
F. prausnitzii
with the multiplicity of infection ratio of 1 and 10 and the EV concentration of 1 μg/mL and 50 μg/mL, respectively. After 24 h, the serotonin level was quantified using an ELISA kit and also the gene expression of serotonin system-related genes was examined using the quantitative real-time PCR method. According to the results, treatment with
A. muciniphila
and
F. prausnitzii
-derived EVs increased the serotonin level, while none of the bacteria could affect the serotonin level in the Caco-2 cells. Both bacteria had significant effects on the mRNA expression of serotonin system-related genes in the Caco-2 cells. Moreover, we observed that
A. muciniphila
and
F. prausnitzii
-derived EVs could impact the expression of major genes involved in the serotonin system. Our findings showed that
A. muciniphila
and
F. prausnitzii
along with their EVs could modulate serotonin system-related genes; hence, they may be useful in microbiota modulation therapies to maintain the homeostasis of the serotonin system. |
doi_str_mv | 10.1007/s12602-021-09786-4 |
format | article |
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Akkermansia muciniphila
and
Faecalibacterium prausnitzii
along with their extracellular vesicles (EVs) on serotonin system-related genes in human epithelial colorectal adenocarcinoma (Caco-2) cells. The differentiated Caco-2 cells were treated with
A. muciniphila
and
F. prausnitzii
with the multiplicity of infection ratio of 1 and 10 and the EV concentration of 1 μg/mL and 50 μg/mL, respectively. After 24 h, the serotonin level was quantified using an ELISA kit and also the gene expression of serotonin system-related genes was examined using the quantitative real-time PCR method. According to the results, treatment with
A. muciniphila
and
F. prausnitzii
-derived EVs increased the serotonin level, while none of the bacteria could affect the serotonin level in the Caco-2 cells. Both bacteria had significant effects on the mRNA expression of serotonin system-related genes in the Caco-2 cells. Moreover, we observed that
A. muciniphila
and
F. prausnitzii
-derived EVs could impact the expression of major genes involved in the serotonin system. Our findings showed that
A. muciniphila
and
F. prausnitzii
along with their EVs could modulate serotonin system-related genes; hence, they may be useful in microbiota modulation therapies to maintain the homeostasis of the serotonin system.</description><identifier>ISSN: 1867-1306</identifier><identifier>EISSN: 1867-1314</identifier><identifier>DOI: 10.1007/s12602-021-09786-4</identifier><identifier>PMID: 33852147</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Adenocarcinoma ; Akkermansia ; Akkermansia muciniphila ; Applied Microbiology ; Caco-2 Cells ; Cell differentiation ; Chemistry ; Chemistry and Materials Science ; Chemistry/Food Science ; Epithelial cells ; Extracellular Vesicles ; Faecalibacterium prausnitzii ; Gastrointestinal tract ; Gene expression ; Homeostasis ; Humans ; Inhibitor drugs ; Intestinal microflora ; Intestine ; Microbiology ; Microbiota ; Multiplicity of infection ; Nutrition ; Protein Science ; Serotonin ; Serotonin - metabolism</subject><ispartof>Probiotics and antimicrobial proteins, 2021-12, Vol.13 (6), p.1546-1556</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021</rights><rights>2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-a5760c2148ee212ea0e8d99e7dc2941c0ce6c9efd5521f083dd9cefd410a9dfe3</citedby><cites>FETCH-LOGICAL-c375t-a5760c2148ee212ea0e8d99e7dc2941c0ce6c9efd5521f083dd9cefd410a9dfe3</cites><orcidid>0000-0002-2043-8523 ; 0000-0001-9514-7571</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33852147$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yaghoubfar, Rezvan</creatorcontrib><creatorcontrib>Behrouzi, Ava</creatorcontrib><creatorcontrib>Zare Banadkoki, Ehsan</creatorcontrib><creatorcontrib>Ashrafian, Fatemeh</creatorcontrib><creatorcontrib>Lari, Arezou</creatorcontrib><creatorcontrib>Vaziri, Farzam</creatorcontrib><creatorcontrib>Nojoumi, Seyed Ali</creatorcontrib><creatorcontrib>Fateh, Abolfazl</creatorcontrib><creatorcontrib>Khatami, Shohreh</creatorcontrib><creatorcontrib>Siadat, Seyed Davar</creatorcontrib><title>Effect of Akkermansia muciniphila, Faecalibacterium prausnitzii, and Their Extracellular Vesicles on the Serotonin System in Intestinal Epithelial Cells</title><title>Probiotics and antimicrobial proteins</title><addtitle>Probiotics & Antimicro. Prot</addtitle><addtitle>Probiotics Antimicrob Proteins</addtitle><description>The gastrointestinal (GI) tract is an essential reservoir of serotonin or 5-hydroxytryptamine (5-HT), which possesses a set of bacterial species communities. Intestinal microbiota has the ability to modulate the host’s serotonin system. In this regard, we evaluated the effect of
Akkermansia muciniphila
and
Faecalibacterium prausnitzii
along with their extracellular vesicles (EVs) on serotonin system-related genes in human epithelial colorectal adenocarcinoma (Caco-2) cells. The differentiated Caco-2 cells were treated with
A. muciniphila
and
F. prausnitzii
with the multiplicity of infection ratio of 1 and 10 and the EV concentration of 1 μg/mL and 50 μg/mL, respectively. After 24 h, the serotonin level was quantified using an ELISA kit and also the gene expression of serotonin system-related genes was examined using the quantitative real-time PCR method. According to the results, treatment with
A. muciniphila
and
F. prausnitzii
-derived EVs increased the serotonin level, while none of the bacteria could affect the serotonin level in the Caco-2 cells. Both bacteria had significant effects on the mRNA expression of serotonin system-related genes in the Caco-2 cells. Moreover, we observed that
A. muciniphila
and
F. prausnitzii
-derived EVs could impact the expression of major genes involved in the serotonin system. Our findings showed that
A. muciniphila
and
F. prausnitzii
along with their EVs could modulate serotonin system-related genes; hence, they may be useful in microbiota modulation therapies to maintain the homeostasis of the serotonin system.</description><subject>Adenocarcinoma</subject><subject>Akkermansia</subject><subject>Akkermansia muciniphila</subject><subject>Applied Microbiology</subject><subject>Caco-2 Cells</subject><subject>Cell differentiation</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemistry/Food Science</subject><subject>Epithelial cells</subject><subject>Extracellular Vesicles</subject><subject>Faecalibacterium prausnitzii</subject><subject>Gastrointestinal tract</subject><subject>Gene expression</subject><subject>Homeostasis</subject><subject>Humans</subject><subject>Inhibitor drugs</subject><subject>Intestinal microflora</subject><subject>Intestine</subject><subject>Microbiology</subject><subject>Microbiota</subject><subject>Multiplicity of infection</subject><subject>Nutrition</subject><subject>Protein Science</subject><subject>Serotonin</subject><subject>Serotonin - metabolism</subject><issn>1867-1306</issn><issn>1867-1314</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kc1O3DAUha2qVaHQF2BRWeqGBYFrO4mTJRoNLRISC362lnFuOgbHSW1HYniSPm49HX4kFl35WP7u8T06hBwwOGYA8iQyXgMvgLMCWtnURfmB7LKmlgUTrPz4qqHeIV9ivAeoa8HhM9kRoqk4K-Uu-bPsezSJjj09fXjAMGgfrabDbKy308o6fUTPNBrt7J02CYOdBzoFPUdv05O1R1T7jl6v0Aa6fExBG3RudjrQW4zWOIx09DStkF5hGNPoradX65hwoFmd-4QxWa8dXU42U85mucgWcZ986rWL-PX53CM3Z8vrxc_i4vLH-eL0ojBCVqnQlazB5CwNImccNWDTtS3KzvC2ZAYM1qbFvqty4B4a0XWtydeSgW67HsUeOdz6TmH8Pedt1GDjJoT2OM5R8YpxKQSvmox-f4fej3PIy2-othIgy1Jmim8pE8YYA_ZqCnbQYa0YqE1vatubyr2pf72pMg99e7ae7wbsXkdeisqA2AIxP_lfGN7-_o_tX40Dpjs</recordid><startdate>20211201</startdate><enddate>20211201</enddate><creator>Yaghoubfar, Rezvan</creator><creator>Behrouzi, Ava</creator><creator>Zare Banadkoki, Ehsan</creator><creator>Ashrafian, Fatemeh</creator><creator>Lari, Arezou</creator><creator>Vaziri, Farzam</creator><creator>Nojoumi, Seyed Ali</creator><creator>Fateh, Abolfazl</creator><creator>Khatami, Shohreh</creator><creator>Siadat, Seyed Davar</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2043-8523</orcidid><orcidid>https://orcid.org/0000-0001-9514-7571</orcidid></search><sort><creationdate>20211201</creationdate><title>Effect of Akkermansia muciniphila, Faecalibacterium prausnitzii, and Their Extracellular Vesicles on the Serotonin System in Intestinal Epithelial Cells</title><author>Yaghoubfar, Rezvan ; Behrouzi, Ava ; Zare Banadkoki, Ehsan ; Ashrafian, Fatemeh ; Lari, Arezou ; Vaziri, Farzam ; Nojoumi, Seyed Ali ; Fateh, Abolfazl ; Khatami, Shohreh ; Siadat, Seyed Davar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-a5760c2148ee212ea0e8d99e7dc2941c0ce6c9efd5521f083dd9cefd410a9dfe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adenocarcinoma</topic><topic>Akkermansia</topic><topic>Akkermansia muciniphila</topic><topic>Applied Microbiology</topic><topic>Caco-2 Cells</topic><topic>Cell differentiation</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chemistry/Food Science</topic><topic>Epithelial cells</topic><topic>Extracellular Vesicles</topic><topic>Faecalibacterium prausnitzii</topic><topic>Gastrointestinal tract</topic><topic>Gene expression</topic><topic>Homeostasis</topic><topic>Humans</topic><topic>Inhibitor drugs</topic><topic>Intestinal microflora</topic><topic>Intestine</topic><topic>Microbiology</topic><topic>Microbiota</topic><topic>Multiplicity of infection</topic><topic>Nutrition</topic><topic>Protein Science</topic><topic>Serotonin</topic><topic>Serotonin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yaghoubfar, Rezvan</creatorcontrib><creatorcontrib>Behrouzi, Ava</creatorcontrib><creatorcontrib>Zare Banadkoki, Ehsan</creatorcontrib><creatorcontrib>Ashrafian, Fatemeh</creatorcontrib><creatorcontrib>Lari, Arezou</creatorcontrib><creatorcontrib>Vaziri, Farzam</creatorcontrib><creatorcontrib>Nojoumi, Seyed Ali</creatorcontrib><creatorcontrib>Fateh, Abolfazl</creatorcontrib><creatorcontrib>Khatami, Shohreh</creatorcontrib><creatorcontrib>Siadat, Seyed Davar</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>ProQuest Biological Science Journals</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Probiotics and antimicrobial proteins</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yaghoubfar, Rezvan</au><au>Behrouzi, Ava</au><au>Zare Banadkoki, Ehsan</au><au>Ashrafian, Fatemeh</au><au>Lari, Arezou</au><au>Vaziri, Farzam</au><au>Nojoumi, Seyed Ali</au><au>Fateh, Abolfazl</au><au>Khatami, Shohreh</au><au>Siadat, Seyed Davar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Akkermansia muciniphila, Faecalibacterium prausnitzii, and Their Extracellular Vesicles on the Serotonin System in Intestinal Epithelial Cells</atitle><jtitle>Probiotics and antimicrobial proteins</jtitle><stitle>Probiotics & Antimicro. Prot</stitle><addtitle>Probiotics Antimicrob Proteins</addtitle><date>2021-12-01</date><risdate>2021</risdate><volume>13</volume><issue>6</issue><spage>1546</spage><epage>1556</epage><pages>1546-1556</pages><issn>1867-1306</issn><eissn>1867-1314</eissn><abstract>The gastrointestinal (GI) tract is an essential reservoir of serotonin or 5-hydroxytryptamine (5-HT), which possesses a set of bacterial species communities. Intestinal microbiota has the ability to modulate the host’s serotonin system. In this regard, we evaluated the effect of
Akkermansia muciniphila
and
Faecalibacterium prausnitzii
along with their extracellular vesicles (EVs) on serotonin system-related genes in human epithelial colorectal adenocarcinoma (Caco-2) cells. The differentiated Caco-2 cells were treated with
A. muciniphila
and
F. prausnitzii
with the multiplicity of infection ratio of 1 and 10 and the EV concentration of 1 μg/mL and 50 μg/mL, respectively. After 24 h, the serotonin level was quantified using an ELISA kit and also the gene expression of serotonin system-related genes was examined using the quantitative real-time PCR method. According to the results, treatment with
A. muciniphila
and
F. prausnitzii
-derived EVs increased the serotonin level, while none of the bacteria could affect the serotonin level in the Caco-2 cells. Both bacteria had significant effects on the mRNA expression of serotonin system-related genes in the Caco-2 cells. Moreover, we observed that
A. muciniphila
and
F. prausnitzii
-derived EVs could impact the expression of major genes involved in the serotonin system. Our findings showed that
A. muciniphila
and
F. prausnitzii
along with their EVs could modulate serotonin system-related genes; hence, they may be useful in microbiota modulation therapies to maintain the homeostasis of the serotonin system.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>33852147</pmid><doi>10.1007/s12602-021-09786-4</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-2043-8523</orcidid><orcidid>https://orcid.org/0000-0001-9514-7571</orcidid></addata></record> |
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language | eng |
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subjects | Adenocarcinoma Akkermansia Akkermansia muciniphila Applied Microbiology Caco-2 Cells Cell differentiation Chemistry Chemistry and Materials Science Chemistry/Food Science Epithelial cells Extracellular Vesicles Faecalibacterium prausnitzii Gastrointestinal tract Gene expression Homeostasis Humans Inhibitor drugs Intestinal microflora Intestine Microbiology Microbiota Multiplicity of infection Nutrition Protein Science Serotonin Serotonin - metabolism |
title | Effect of Akkermansia muciniphila, Faecalibacterium prausnitzii, and Their Extracellular Vesicles on the Serotonin System in Intestinal Epithelial Cells |
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