3D printed vascular phantoms for high-resolution biophotonic image quality assessment via direct laser writing

Fluorescence imaging techniques such as fluorescein angiography and fundus autofluorescence are often used to diagnose retinal pathologies; however, there are currently no standardized test methods for evaluating device performance. Here we present microstructured fluorescent phantoms fabricated usi...

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Bibliographic Details
Published in:Optics letters 2021-04, Vol.46 (8), p.1987-1990
Main Authors: Horng, Hannah, O'Brien, Kerry, Lamont, Andrew, Sochol, Ryan D, Pfefer, T Joshua, Chen, Yu
Format: Article
Language:English
Subjects:
Angiography
Biomimetics
Broadband
Diameters
Direct laser writing
Fluorescein
Fluorescence
Fluorescent dyes
Image quality
Image resolution
Imaging techniques
Microfluidics
Performance evaluation
Photoresists
Quality assessment
Three dimensional printing
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Summary:Fluorescence imaging techniques such as fluorescein angiography and fundus autofluorescence are often used to diagnose retinal pathologies; however, there are currently no standardized test methods for evaluating device performance. Here we present microstructured fluorescent phantoms fabricated using a submicron-scale three-dimensional printing technology, direct laser writing (DLW). We employ an in situ DLW technique to print 10 µm diameter microfluidic channels that support perfusions of fluorescent dyes. We then demonstrate how broadband photoresist fluorescence can be exploited to generate resolution targets and biomimetic models of retinal vasculature using standard DLW processes. The results indicate that these approaches show significant promise for generating better performance evaluation tools for fluorescence microscopy and imaging devices.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.412849