Annexins A1 and A2 Accumulate and Are Immobilized at Cross-Linked Membrane–Membrane Interfaces

• Published in: Biochemistry (Easton) 2021-04, Vol.60 (16), p.1248-1259
• Main Authors: Berg Klenow, Martin, Iversen, Christoffer, Wendelboe Lund, Frederik, Mularski, Anna, Busk Heitmann, Anne Sofie, Dias, Catarina, Nylandsted, Jesper, Simonsen, Adam Cohen
• Format: Article
• Language: English
• Subjects: Annexin A1 - chemistry; Annexin A1 - metabolism; Annexin A2 - chemistry; Annexin A2 - metabolism; Cell Membrane - metabolism; Cross-Linking Reagents - chemistry; Cross-Linking Reagents - metabolism; Fluorescence Recovery After Photobleaching; Humans; Immobilized Proteins - chemistry; Immobilized Proteins - metabolism; Phosphatidylserines - chemistry; Phosphatidylserines - metabolism; Unilamellar Liposomes - chemistry; Unilamellar Liposomes - metabolism
• ISSN: 0006-2960; 1520-4995; 1520-4995
• DOI: 10.1021/acs.biochem.1c00126

Rapid membrane repair is required to ensure cell survival after rupture of the plasma membrane. The annexin family of proteins is involved in plasma membrane repair (PMR) and is activated by the influx of Ca2+ from the extracellular medium at the site of injury. Annexins A1 and A2 (ANXA1 and ANXA2, respectively) are structurally similar and bind to negatively charged phosphatidylserine (PS) to induce membrane cross-linking and to promote fusion, which are both essential processes that occur during membrane repair. The degree of annexin accumulation and the annexin mobility at cross-linked membranes are important aspects of ANXA1 and ANXA2 function in repair. Here, we quantify ANXA1- and ANXA2-induced membrane cross-linking between giant unilamellar vesicles (GUVs). Time-lapse measurements show that ANXA1 and ANXA2 can induce membrane cross-linking on a time scale compatible with PMR. Cross-linked membrane–membrane interfaces between the GUVs persist in time without fusion, and quantification of confocal microscopy images demonstrates that ANXA1, ANXA2, and, to a lesser extent, PS lipids accumulate at the double membrane interface. Fluorescence recovery after photobleaching shows that the annexins are fully immobilized at the double membrane interface, whereas PS lipids display a 75% decrease in mobility. In addition, the complete immobilization of annexins between two membranes indicates a high degree of network formation between annexins, suggesting that membrane cross-linking is mainly driven by protein–protein interactions.