Selective colorimetric urine glucose detection by paper sensor functionalized with polyaniline nanoparticles and cell membrane

• Published in: Analytica chimica acta 2021-05, Vol.1158, p.338387-338387, Article 338387
• Main Authors: Lee, Taeha, Kim, Insu, Cheong, Da Yeon, Roh, Seokbeom, Jung, Hyo Gi, Lee, Sang Won, Kim, Hyun Soo, Yoon, Dae Sung, Hong, Yoochan, Lee, Gyudo
• Format: Article
• Language: English
• Subjects: Aniline Compounds; Blood Glucose; Blood Glucose Self-Monitoring; Colorimetric paper sensor; Colorimetry; Glucose; High selectivity; Nanoparticles; Noninvasive; Polyaniline nanoparticles; Red blood cell membrane; Urine glucose
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2021.338387

For the diabetes diagnosis, noninvasive methods are preferred to invasive methods; urine glucose measurement is an example of a noninvasive method. However, conventional noninvasive methods for urine glucose measurement are not intuitive. Furthermore, such methods exhibit low selectivity because they can detect interfering molecules in addition to glucose. Herein, we fabricate a noninvasive, intuitive, and highly selective paper sensor consisting of polyaniline nanoparticles (PAni-NPs) and red blood cell membranes (RBCMs). The PAni-NPs (adsorbed on the paper) are highly sensitive to hydrogen ions and change color from emeraldine blue to emeraldine green within a few seconds. The RBCM (coated on the PAni-NP-adsorbed paper) having the glucose transporter-1 protein plays the role of a smart filter that transports glucose but rejects other interfering molecules. In particular, the selectivity of the RBCM-coated PAni-NP-based paper sensor was approximately improved ∼85%, compared to the uncoated paper sensors. The paper sensor could detect urine glucose over the range of 0–10 mg/mL (0–56 mM), with a limit of detection of 0.54 mM. The proposed paper sensor will facilitate the development of a highly selective and colorimetric urine glucose monitoring system. [Display omitted] •A highly selective paper-based sensor was fabricated for noninvasive urine glucose detection.•PAni-NP-functionalized cellulose paper was coated with a cell membrane to enhance selectivity.•RBCM prevents interfering molecules from approaching GOx, whereas GLUT-1 in RBCM selectively transports glucose to GOx.•PAni and RPAni paper sensors can be a useful technique for early detection of diabetes and rapid screening of glucose.