Functional analysis and expression patterns of members of the FLOWERING LOCUS T (FT) gene family in Lilium

• Published in: Plant physiology and biochemistry 2021-06, Vol.163, p.250-260
• Main Authors: Yan, Xiao, Cao, Qin-Zheng, He, Heng-Bin, Wang, Lian-Juan, Jia, Gui-Xia
• Format: Article
• Language: English
• Subjects: Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Bulblet differentiation; Floral induction; Flowers - genetics; Flowers - metabolism; Gene Expression Regulation, Plant; Gene family; LFTs; Lilium - genetics; Lilium - metabolism; Photoperiod; Phylogeny; Plant Proteins - genetics; Plant Proteins - metabolism; Plants, Genetically Modified - genetics; Plants, Genetically Modified - metabolism
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2021.03.056

Lilium is an important commercial flowering species, and there are many varieties and more than 100 species of wild Lilium. Lilium × formolongi is usually propagated from seedlings, and the flowering of these plants is driven mainly by the photoperiodic pathway. Most of the other lily plants are propagated via bulblets and need to be vernalized; these plants can be simply divided into pretransplantation types and posttransplantation types according to the time at which the floral transition occurs. We identified three Lilium FLOWERING LOCUS T (LFT) family members in 7 Lilium varieties, and for each gene, the coding sequence of the different varieties was identical. Among these genes, the LFT1 gene of Lilium was most homologous to the AtFT gene, which promotes flowering in Arabidopsis. We analyzed the expression patterns of LFT genes in Lilium × formolongi seedlings and in different Lilium varieties, and the results showed that LFT1 and LFT3 may promote floral induction. Compared with LFT3, LFT1 may have a greater effect on floral induction in Lilium, which is photoperiod sensitive, while LFT3 may play a more important role in the floral transition of lily plants, which have a high requirement for vernalization. LFT2 may be involved in the differentiation of bulblets, which was verified by tissue culture experiments, and LFT1 may have other functions involved in promoting bulblet growth. The functions of LFT genes were verified by the use of transgenic Arabidopsis thaliana plants, which showed that both the LFT1 and LFT3 genes can promote early flowering in Arabidopsis. Compared with LFT3, LFT1 promoted flowering more obviously, and thus, this gene could be an important promoter of floral induction in Lilium. •Coding sequences of the LFT gene family members in different varieties were identical•During the floral transition of Lilium, LFT1 may have a greater effect on photoperiod sensitive Lilium, while LFT3 may be more important in Lilium which have higher requirements for vernalization•LFT2 may be involved in the differentiation and formation of bulblets•Both the LFT1 and LFT3 can promote early flowering in Arabidopsis