Inter- and Intra-Isolate rRNA Large Subunit Variation in Glomus coronatum Spores

High levels of variation are reported in the large subunit (LSU) rRNA gene, D2 region of Glomus coronatum, a well characterized species of arbuscular mycorrhizal fungus (AMF). Clones (435) containing the D2 regions from 7 isolates of G. coronatum were investigated for intra- and inter-isolate sequen...

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Bibliographic Details
Published in:The New phytologist 2001-03, Vol.149 (3), p.539-554
Main Authors: Clapp, J. P., Rodriguez, A., Dodd, J. C.
Format: Article
Language:English
Subjects:
Biodiversity
Biological and medical sciences
DNA
Fundamental and applied biological sciences. Psychology
Fungal spores
Fungi
Gels
Genetic variation
Glomus constrictum
Glomus coronatum
Glomus mosseae
Mycorrhizal fungi
Parasitism and symbiosis
Plant cytology, morphology, systematics, chorology and evolution
Plant physiology and development
Polymerase chain reaction
rRNA large subunit
Species
Spores
SSCP
Symbiosis
Taxa
Thallophyta
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Summary:High levels of variation are reported in the large subunit (LSU) rRNA gene, D2 region of Glomus coronatum, a well characterized species of arbuscular mycorrhizal fungus (AMF). Clones (435) containing the D2 regions from 7 isolates of G. coronatum were investigated for intra- and inter-isolate sequence variation using PCR-single-strand conformational polymorphism (PCR-SSCP) as a prescreen before sequencing. Isolates of G. mosseae, G. constrictum and G. geosporum, three species of AMF with similar spore ontogeny and morphology, were also analysed. Analysis of 138 representative sequences indicated that most were unique; this variation could not be attributed to DNA polymerase or cloning artefacts. Only 13 sequences were found in more than one isolate. Neighbour-joining analysis showed that most sequences from G. coronatum formed a main group although several sequences from G. mosseae and G. constrictum clustered with G. coronatum. There was greater than expected variation in the LSU D2 region sequences from G. coronatum. The four Glomus species, closely related by spore morphology, might represent part of a genetic continuum. Implications for the concept of species in AMF, the use of rRNA sequences to estimate biodiversity and in situ detection in field ecology are discussed.
ISSN:0028-646X
1469-8137
DOI:10.1046/j.1469-8137.2001.00060.x