Quantitative Fluorescence Resonance Energy Transfer Analysis on the Direct Interaction of Activation-2b with Histone H3/Switch-3B Protein in Arabidopsis Mesophyll Protoplasts

Interaction between the alteration/deficiency in activation-2b (ADA2b) and histone H3/switch-3B (SWI3B) proteins was evaluated in arabidopsis mesophyll protoplasts by quantitative fluorescence resonance energy transfer (FRET) analysis. Microscopic image showed that ADA2b, SWI3B and H3 proteins coloc...

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Bibliographic Details
Published in:Journal of fluorescence 2021-07, Vol.31 (4), p.981-988
Main Authors: Gao, Lu, Lin, Fangrui, Han, Danlu, Jiang, Jieming, Yang, Chengwei, Zhuang, Zhengfei, Chen, Tongsheng
Format: Article
Language:English
Subjects:
Analytical Chemistry
Arabidopsis - metabolism
Arabidopsis Proteins - metabolism
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biomedicine
Biophysics
Biotechnology
Energy transfer
Fluorescence
Fluorescence Resonance Energy Transfer
Histones
Histones - metabolism
Mesophyll Cells - metabolism
Original Article
Protein Binding
Proteins
Protoplasts
Protoplasts - metabolism
Resonance
Transcription Factors - metabolism
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Summary:Interaction between the alteration/deficiency in activation-2b (ADA2b) and histone H3/switch-3B (SWI3B) proteins was evaluated in arabidopsis mesophyll protoplasts by quantitative fluorescence resonance energy transfer (FRET) analysis. Microscopic image showed that ADA2b, SWI3B and H3 proteins colocalized in nucleus, and quantitative FRET measurements showed 0.31 of FRET efficiency ( E ) for the protoplasts coexpressing ECFP-ADA2b and EYFP-SWI3B, and 0.285 of E for the protoplasts coexpressing ECFP-H3 and EYFP-ADA2b, demonstrating the direct interaction of ADA2b with SWI3B/H3 protein. Collectively, SWI3B and H3 proteins are the inherent components of the ADA2b complex in which ADA2b directly interacts with SWI3B/H3 protein.
ISSN:1053-0509
1573-4994
1573-4994
DOI:10.1007/s10895-021-02728-x