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Quantitative Fluorescence Resonance Energy Transfer Analysis on the Direct Interaction of Activation-2b with Histone H3/Switch-3B Protein in Arabidopsis Mesophyll Protoplasts
Interaction between the alteration/deficiency in activation-2b (ADA2b) and histone H3/switch-3B (SWI3B) proteins was evaluated in arabidopsis mesophyll protoplasts by quantitative fluorescence resonance energy transfer (FRET) analysis. Microscopic image showed that ADA2b, SWI3B and H3 proteins coloc...
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| Published in: | Journal of fluorescence 2021-07, Vol.31 (4), p.981-988 |
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| Main Authors: | , , , , , , |
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| container_end_page | 988 |
| container_issue | 4 |
| container_start_page | 981 |
| container_title | Journal of fluorescence |
| container_volume | 31 |
| creator | Gao, Lu Lin, Fangrui Han, Danlu Jiang, Jieming Yang, Chengwei Zhuang, Zhengfei Chen, Tongsheng |
| description | Interaction between the alteration/deficiency in activation-2b (ADA2b) and histone H3/switch-3B (SWI3B) proteins was evaluated in arabidopsis mesophyll protoplasts by quantitative fluorescence resonance energy transfer (FRET) analysis. Microscopic image showed that ADA2b, SWI3B and H3 proteins colocalized in nucleus, and quantitative FRET measurements showed 0.31 of FRET efficiency (
E
) for the protoplasts coexpressing ECFP-ADA2b and EYFP-SWI3B, and 0.285 of
E
for the protoplasts coexpressing ECFP-H3 and EYFP-ADA2b, demonstrating the direct interaction of ADA2b with SWI3B/H3 protein. Collectively, SWI3B and H3 proteins are the inherent components of the ADA2b complex in which ADA2b directly interacts with SWI3B/H3 protein. |
| doi_str_mv | 10.1007/s10895-021-02728-x |
| format | article |
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E
) for the protoplasts coexpressing ECFP-ADA2b and EYFP-SWI3B, and 0.285 of
E
for the protoplasts coexpressing ECFP-H3 and EYFP-ADA2b, demonstrating the direct interaction of ADA2b with SWI3B/H3 protein. Collectively, SWI3B and H3 proteins are the inherent components of the ADA2b complex in which ADA2b directly interacts with SWI3B/H3 protein.</description><identifier>ISSN: 1053-0509</identifier><identifier>ISSN: 1573-4994</identifier><identifier>EISSN: 1573-4994</identifier><identifier>DOI: 10.1007/s10895-021-02728-x</identifier><identifier>PMID: 33880705</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Analytical Chemistry ; Arabidopsis - metabolism ; Arabidopsis Proteins - metabolism ; Biochemistry ; Biological and Medical Physics ; Biomedical and Life Sciences ; Biomedicine ; Biophysics ; Biotechnology ; Energy transfer ; Fluorescence ; Fluorescence Resonance Energy Transfer ; Histones ; Histones - metabolism ; Mesophyll Cells - metabolism ; Original Article ; Protein Binding ; Proteins ; Protoplasts ; Protoplasts - metabolism ; Resonance ; Transcription Factors - metabolism</subject><ispartof>Journal of fluorescence, 2021-07, Vol.31 (4), p.981-988</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c326t-ab9eb75e8df6c6cab0f2f5daa3075a662ea102c88fa892994e3b8d0392db26183</cites><orcidid>0000-0002-9036-6676</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33880705$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gao, Lu</creatorcontrib><creatorcontrib>Lin, Fangrui</creatorcontrib><creatorcontrib>Han, Danlu</creatorcontrib><creatorcontrib>Jiang, Jieming</creatorcontrib><creatorcontrib>Yang, Chengwei</creatorcontrib><creatorcontrib>Zhuang, Zhengfei</creatorcontrib><creatorcontrib>Chen, Tongsheng</creatorcontrib><title>Quantitative Fluorescence Resonance Energy Transfer Analysis on the Direct Interaction of Activation-2b with Histone H3/Switch-3B Protein in Arabidopsis Mesophyll Protoplasts</title><title>Journal of fluorescence</title><addtitle>J Fluoresc</addtitle><addtitle>J Fluoresc</addtitle><description>Interaction between the alteration/deficiency in activation-2b (ADA2b) and histone H3/switch-3B (SWI3B) proteins was evaluated in arabidopsis mesophyll protoplasts by quantitative fluorescence resonance energy transfer (FRET) analysis. Microscopic image showed that ADA2b, SWI3B and H3 proteins colocalized in nucleus, and quantitative FRET measurements showed 0.31 of FRET efficiency (
E
) for the protoplasts coexpressing ECFP-ADA2b and EYFP-SWI3B, and 0.285 of
E
for the protoplasts coexpressing ECFP-H3 and EYFP-ADA2b, demonstrating the direct interaction of ADA2b with SWI3B/H3 protein. Collectively, SWI3B and H3 proteins are the inherent components of the ADA2b complex in which ADA2b directly interacts with SWI3B/H3 protein.</description><subject>Analytical Chemistry</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Biochemistry</subject><subject>Biological and Medical Physics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Biophysics</subject><subject>Biotechnology</subject><subject>Energy transfer</subject><subject>Fluorescence</subject><subject>Fluorescence Resonance Energy Transfer</subject><subject>Histones</subject><subject>Histones - metabolism</subject><subject>Mesophyll Cells - metabolism</subject><subject>Original Article</subject><subject>Protein Binding</subject><subject>Proteins</subject><subject>Protoplasts</subject><subject>Protoplasts - metabolism</subject><subject>Resonance</subject><subject>Transcription Factors - metabolism</subject><issn>1053-0509</issn><issn>1573-4994</issn><issn>1573-4994</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kc9u1DAQxi0EoqXwAhyQJS5cQsd2nDjHpbRspSL-lXPkOJOuq6yd2k7pvhTPiNMtIHFAsuWZ8W--keYj5CWDtwygPo4MVCML4Czfmqvi7hE5ZLIWRdk05eMcgxQFSGgOyLMYrwGgUaV6Sg6EUApqkIfk55dZu2STTvYW6dk4-4DRoDNIv2L0Ti_RqcNwtaOXQbs4YKArp8ddtJF6R9MG6Xsb0CR67hIGbZLNZT_QVY5u9ZIVvKM_bNrQtY3JO6RrcfwtF8ymEO_o5-ATWkfzWQXd2d5Pi_bHPH7a7MbxHvDTqGOKz8mTQY8RXzy8R-T72enlybq4-PTh_GR1URjBq1TorsGulqj6oTKV0R0MfJC91gJqqauKo2bAjVKDVg3Py0LRqR5Ew_uOV0yJI_JmrzsFfzNjTO3W5rWMo3bo59hyySrORVnKjL7-B732c8gbWqiSKVkDKzPF95QJPsaAQzsFu9Vh1zJoFzfbvZttdrO9d7O9y02vHqTnbov9n5bf9mVA7IGYv9wVhr-z_yP7C0Emrks</recordid><startdate>20210701</startdate><enddate>20210701</enddate><creator>Gao, Lu</creator><creator>Lin, Fangrui</creator><creator>Han, Danlu</creator><creator>Jiang, Jieming</creator><creator>Yang, Chengwei</creator><creator>Zhuang, Zhengfei</creator><creator>Chen, Tongsheng</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9036-6676</orcidid></search><sort><creationdate>20210701</creationdate><title>Quantitative Fluorescence Resonance Energy Transfer Analysis on the Direct Interaction of Activation-2b with Histone H3/Switch-3B Protein in Arabidopsis Mesophyll Protoplasts</title><author>Gao, Lu ; Lin, Fangrui ; Han, Danlu ; Jiang, Jieming ; Yang, Chengwei ; Zhuang, Zhengfei ; Chen, Tongsheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-ab9eb75e8df6c6cab0f2f5daa3075a662ea102c88fa892994e3b8d0392db26183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Analytical Chemistry</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Biochemistry</topic><topic>Biological and Medical Physics</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Biophysics</topic><topic>Biotechnology</topic><topic>Energy transfer</topic><topic>Fluorescence</topic><topic>Fluorescence Resonance Energy Transfer</topic><topic>Histones</topic><topic>Histones - metabolism</topic><topic>Mesophyll Cells - metabolism</topic><topic>Original Article</topic><topic>Protein Binding</topic><topic>Proteins</topic><topic>Protoplasts</topic><topic>Protoplasts - metabolism</topic><topic>Resonance</topic><topic>Transcription Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gao, Lu</creatorcontrib><creatorcontrib>Lin, Fangrui</creatorcontrib><creatorcontrib>Han, Danlu</creatorcontrib><creatorcontrib>Jiang, Jieming</creatorcontrib><creatorcontrib>Yang, Chengwei</creatorcontrib><creatorcontrib>Zhuang, Zhengfei</creatorcontrib><creatorcontrib>Chen, Tongsheng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of fluorescence</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gao, Lu</au><au>Lin, Fangrui</au><au>Han, Danlu</au><au>Jiang, Jieming</au><au>Yang, Chengwei</au><au>Zhuang, Zhengfei</au><au>Chen, Tongsheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative Fluorescence Resonance Energy Transfer Analysis on the Direct Interaction of Activation-2b with Histone H3/Switch-3B Protein in Arabidopsis Mesophyll Protoplasts</atitle><jtitle>Journal of fluorescence</jtitle><stitle>J Fluoresc</stitle><addtitle>J Fluoresc</addtitle><date>2021-07-01</date><risdate>2021</risdate><volume>31</volume><issue>4</issue><spage>981</spage><epage>988</epage><pages>981-988</pages><issn>1053-0509</issn><issn>1573-4994</issn><eissn>1573-4994</eissn><abstract>Interaction between the alteration/deficiency in activation-2b (ADA2b) and histone H3/switch-3B (SWI3B) proteins was evaluated in arabidopsis mesophyll protoplasts by quantitative fluorescence resonance energy transfer (FRET) analysis. Microscopic image showed that ADA2b, SWI3B and H3 proteins colocalized in nucleus, and quantitative FRET measurements showed 0.31 of FRET efficiency (
E
) for the protoplasts coexpressing ECFP-ADA2b and EYFP-SWI3B, and 0.285 of
E
for the protoplasts coexpressing ECFP-H3 and EYFP-ADA2b, demonstrating the direct interaction of ADA2b with SWI3B/H3 protein. Collectively, SWI3B and H3 proteins are the inherent components of the ADA2b complex in which ADA2b directly interacts with SWI3B/H3 protein.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>33880705</pmid><doi>10.1007/s10895-021-02728-x</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-9036-6676</orcidid></addata></record> |
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| subjects | Analytical Chemistry Arabidopsis - metabolism Arabidopsis Proteins - metabolism Biochemistry Biological and Medical Physics Biomedical and Life Sciences Biomedicine Biophysics Biotechnology Energy transfer Fluorescence Fluorescence Resonance Energy Transfer Histones Histones - metabolism Mesophyll Cells - metabolism Original Article Protein Binding Proteins Protoplasts Protoplasts - metabolism Resonance Transcription Factors - metabolism |
| title | Quantitative Fluorescence Resonance Energy Transfer Analysis on the Direct Interaction of Activation-2b with Histone H3/Switch-3B Protein in Arabidopsis Mesophyll Protoplasts |
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