The subunit vaccine H65 + CAF01 increased the BCG- protection against Mycobacterium bovis infection in a mouse model of bovine tuberculosis

H65, a fusion protein of three pairs of ESX-secreted antigens of Mycobacterium tuberculosis and Mycobacterium bovis, formulated with the liposomal adjuvant CAF01 has been shown to confer protection against M. tuberculosis infection in mice. In this study, we evaluated the impact of combining BCG wit...

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Bibliographic Details
Published in:Research in veterinary science 2021-05, Vol.136, p.595-597
Main Authors: Blanco, Federico C., García, Elizabeth A., Aagaard, Claus, Bigi, Fabiana
Format: Article
Language:English
Subjects:
Antigens
CAF01
Cattle
Fusion protein
H65
Immunization
Infections
Laboratory animals
Mycobacterium bovis
Proteins
Tuberculosis
Vaccine
Vaccines
Veterinary medicine
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Summary:H65, a fusion protein of three pairs of ESX-secreted antigens of Mycobacterium tuberculosis and Mycobacterium bovis, formulated with the liposomal adjuvant CAF01 has been shown to confer protection against M. tuberculosis infection in mice. In this study, we evaluated the impact of combining BCG with H65 + CAF01 immunization in a M. bovis mouse model of infection. We found that a BCG-H65 + CAF01/ H65 + CAF01 prime-boost scheme induced higher protection than BCG and H65 + CAF01 alone. Altogether, H65 antigen formulated in liposomal adjuvant improved the BCG-induced immune protection, thus making this vaccine strategy a promising tool to control bovine tuberculosis. •H65 fusion protein conferred protection against Mycobacterium bovis challenge in a mouse model.•H65 fusion protein improved the protection conferred by BCG.•BCG/H65 vaccine is compatible with an ESAT-6 / CFP-10 antigen-based diagnostic method.
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2021.04.014