Autoimmune pancytopenia after liver transplantation: A case report

• Published in: Transfusion and apheresis science 2021-08, Vol.60 (4), p.103136-103136, Article 103136
• Main Authors: Barros, Melca Maria Oliveira, Ayoub, Fauze Lutfe, Lemos, Giovanna, Brasileiro, Kelly Cristina, da Silva Icibaci, Priscilla Brito, Moritz, Elyse, Martins, Juliana Oliveira, Bub, Carolina Bonet, Chiba, Akemi Kuroda, Bordin, Jose Orlando
• Format: Article
• Language: English
• Subjects: Autoantibodies - blood; Autoimmune Diseases - blood; Autoimmune Diseases - etiology; Autoimmune Diseases - therapy; Autoimmune hemolytic anemia (AIHA); Autoimmune neutropenia (AIN); Case report; GIFT; Humans; Immune thrombocytopenia (ITP); Immunosuppression Therapy; Liver Transplantation; MAIPA; Male; Middle Aged; Pancytopenia - blood; Pancytopenia - etiology; Pancytopenia - therapy; Prednisolone - administration & dosage
• ISSN: 1473-0502; 1878-1683
• DOI: 10.1016/j.transci.2021.103136

Autoimmune hemolytic anemia (AIHA), immune thrombocytopenia (ITP), and autoimmune neutropenia (AIN) are reported in the literature after liver, intestinal, heart, pancreas, and kidney transplants. We report a case of autoimmune pancytopenia (AIHA, AIN and ITP) 9 years after liver transplantation with confirmed erythrocyte and neutrophil auto-antibodies. A 49 years old man was admitted to our hospital presented with dysentery and fever, with history of liver transplantation in 2008. Laboratory evaluation demonstrated hemoglobin: 7.2 g/dL, granulocytes: 0.10 × 109/L and platelets: 15 × 109/mm³; indirect bilirubin: 3.62 mg/dL; lactate dehydrogenase: 603 U/L. Direct antiglobulin test revealed a monospecific anti-IgG plus C3 and the acid eluate was reactive to all panel red cells, consistent with an AIHA. Granulocyte immunofluorescence test (GIFT) and agglutination test (GAT) were reactive for granulocytes. Test with Luminex technology for human neutrophil antigen (HNA) antibody detection was strong reactive with beads expressing HNA-1a, -1b, -1c, -2, -4a and -5a antigens. HNA genotyping revealed the presence of the corresponding antigens, confirming the autoantibodies. Test with Luminex technology for human leucocyte antigen (HLA) antibody detection was negative. Monoclonal antibody immobilization of platelet antigens (MAIPA) assay was negative. Viral causes were excluded. The condition was compatible with clinical onset of autoimmune pancytopenia. Prednisone was administered at an initial dose of 1 mg/kg/day and immunosuppressive therapy was adjusted. This treatment resulted in rapid resolution of pancytopenia. Combined autoimmune pancytopenia (AIHA, AIN and ITP) is a rare condition that may occur after liver transplantation. Early recognition of this phenomenon permits appropriate treatment.