Analysis of adsorption energy distribution in selected hydrophilic‐interaction chromatography systems with amide, amine, and zwitterionic stationary phases

Adsorption mechanisms of caffeine, quercetin, and phenol as test substances in various chromatographic systems have been analyzed. The investigations were conducted using three different chromatographic columns packed with polar bonded stationary phases, that is, amide, amine, and zwitterionic. Meth...

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Bibliographic Details
Published in:Journal of separation science 2021-07, Vol.44 (13), p.2577-2586
Main Authors: Ziobrowski, Piotr, Chutkowski, Marcin, Przywara, Mateusz, Zapała, Lidia, Kosińska‐Pezda, Małgorzata, Zapała, Wojciech
Format: Article
Language:English
Subjects:
Acetonitrile
Adsorption
Caffeine
Chromatography
energetic heterogeneity
Energy distribution
isotherm
Phases
Scatchard curves
Scatchard plot
Zwitterions
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Summary:Adsorption mechanisms of caffeine, quercetin, and phenol as test substances in various chromatographic systems have been analyzed. The investigations were conducted using three different chromatographic columns packed with polar bonded stationary phases, that is, amide, amine, and zwitterionic. Methanol–water and acetonitrile–water systems with different organic solvent contents have been used as mobile phases. On the basis of adsorption isotherms obtained for the tested systems, Scatchard plots and adsorption energy distributions have been determined. The most likely retention mechanisms have been discussed. The results of investigations indicate that (i) the surfaces of tested adsorbents are energetically heterogeneous, and (ii) the main role in sorption mechanism is played by low‐energy sites.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.202100160