A dual-signal fluorescent probe for detection of acid phosphatase

Acid phosphatase has become a significant indicator of prognostic and medical diagnosis, and its dysfunction may lead to a series of diseases. A novel dual-signal fluorescence method for acid phosphatase detection based on europium polymer (europium-pyridine dicarboxylicacid-adenine) and pyridoxal p...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2021-06, Vol.413 (15), p.3925-3932
Main Authors: Li, Shuangqin, Fu, Guoqing, Wang, Yaya, Xiang, Yueci, Mu, Shuai, Xu, Yixuan, Liu, Xiaoyan, Zhang, Haixia
Format: Article
Language:English
Subjects:
Acid phosphatase
Acids
Addition polymerization
Adenine
Aldehydes
Analysis
Analytical Chemistry
Biochemistry
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Diagnosis
Electron transfer
Emission spectra
Emissions
Europium
Fluorescence
Fluorescent indicators
Food Science
Laboratory Medicine
Methods
Monitoring/Environmental Analysis
Phosphatase
Plant growth
Polyethylene terephthalate
Polymers
Pyridines
Research Paper
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Summary:Acid phosphatase has become a significant indicator of prognostic and medical diagnosis, and its dysfunction may lead to a series of diseases. A novel dual-signal fluorescence method for acid phosphatase detection based on europium polymer (europium-pyridine dicarboxylicacid-adenine) and pyridoxal phosphate (PLP) was proposed. PLP coordinated with europium polymer via Eu 3+ and P–O bonds, and the fluorescence of europium polymer was quenched due to the photoinduced electron transfer (PET) effect between aldehyde and europium polymer. Upon addition of acid phosphatase, the PLP was transformed to phosphate (Pi) and pyridoxal (PL). The PL was released from the surface of europium polymer, and the blue emission was enhanced due to the formation of internal hemiacetal, while the fluorescence of europium polymer recovered. The blue (PL) and red emission (Eu 3+ ) were positively correlated with acid phosphatase activity; thus the sensitive assay of acid phosphatase was effectively achieved. The two signals were applied to determine the acid phosphatase with limits of detection (LOD) of 0.04 mU/mL and 0.38 mU/mL, and the linear ranges were 0.13–5.00 mU/mL and 1.25–20.00 mU/mL, respectively. The probe can be used to trace the acid phosphatase in biological systems and holds promise for use in clinical diagnosis and early prevention. Graphical abstract
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-021-03343-2