Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers on gellan gum films

• Published in: International journal of biological macromolecules 2021-07, Vol.183, p.727-742
• Main Authors: Rufato, Késsily B., Souza, Paulo R., de Oliveira, Ariel C., Berton, Sharise B.R., Sabino, Roberta M., Muniz, Edvani C., Popat, Ketul C., Radovanovic, Eduardo, Kipper, Matt J., Martins, Alessandro F.
• Format: Article
• Language: English
• Subjects: Anti-adhesive coatings; Iota-carrageenan; Polysaccharides
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2021.04.138

In this work free-standing gels formed from gellan gum (GG) by solvent evaporation are coated with polysaccharide-based polyelectrolyte multilayers, using the layer-by-layer approach. We show that PEMs composed of iota-carrageenan (CAR) and three different natural polycationic polymers have composition-dependent antimicrobial properties, and support mammalian cell growth. Cationic polymers (chitosan (CHT), N,N,N-trimethyl chitosan (TMC), and an amino-functionalized tannin derivative (TN)) are individually assembled with the anionic iota-carrageenan (CAR) at pH 5.0. PEMs (15-layers) are alternately deposited on the GG film. The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements. The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm). Polymer coatings promote stability and durability of the GG film, and introduce antimicrobial properties against Gram-negative (Salmonella enteritidis) and Gram-positive (Staphylococcus aureus) bacteria. The films are also cytocompatible. Therefore, they have properties that can be further developed as wound dressings and food packaging. •A commercial gellan gum is characterized, supporting stable films due to the metallic ions presence in its composition.•The solvent evaporation method provides a stiffer gellan gum film.•The film is coated with polyelectrolyte multilayers due to the carboxylate moieties on the gellan gum.•Chitosan/iota-carrageenan and N,N,N-trimethyl chitosan/iota-carrageenan coatings support antimicrobial activities.•The coated films can be applied in the food and biomedical fields.