Poly (L‐lactic acid) nanofibrous scaffolds support the proliferation and neural differentiation of mouse neural stem and progenitor cells

• Published in: International journal of developmental neuroscience 2021-08, Vol.81 (5), p.438-447
• Main Authors: Miri, Vahideh, Asadi, Asadollah, Sagha, Mohsen, Najafzadeh, Nowruz, Golmohammadi, Mohammad Ghasem
• Format: Article
• Language: English
• Subjects: Animals; Cell Differentiation - drug effects; Cell Proliferation - drug effects; GFAP; Glial Fibrillary Acidic Protein - metabolism; Immunohistochemistry; Lateral Ventricles - cytology; Lateral Ventricles - drug effects; Male; MAP2; Materials Testing; Mice; Mice, Inbred BALB C; Microtubule-Associated Proteins - metabolism; Nanofibers; neural stem and progenitor cells; Neural Stem Cells - drug effects; Neuroglia - drug effects; PLLA; Polyesters - pharmacology; scaffold; Stem Cells - drug effects; tissue engineering; Tissue Scaffolds
• ISSN: 0736-5748; 1873-474X
• DOI: 10.1002/jdn.10119

Background The distribution and growth of cells on nanofibrous scaffolds seem to be an indispensable precondition in cell tissue engineering. The potential use of biomaterial scaffolds in neural stem cell therapy is increasingly attracting attention. Aim In this study, we produced porous nanofibrous scaffolds fabricated from random poly‐L‐lactic acid (PLLA) to support neurogenic differentiation of neural stem and progenitor cells (NSPCs), isolated from the subventricular zone (SVZ) of the adult mouse brain. Methods The viability and proliferation of the NSPCs on the nanofibrous PLLA scaffold were also tested by nuclear staining with 4, 6‐diamidino‐2‐phenylindole dihydrochloride (DAPI), 3‐(4, 5‐dimethylthiazol‐2‐yl)‐2, 5‐diphenyl tetrazolium bromide (MTT) assay and scanning electron microscopy (SEM). To investigate the differentiation potential of NSPCs on the scaffolds, the cells were treated with a neurogenic differentiation medium, and immunostaining was done to detect neuronal and glial cells after 14 and 21 days of cultivation. Furthermore, the morphology of differentiated cells on the scaffold was examined using SEM. Results The DAPI staining revealed the proliferation of NSPCs onto the surface of the nanofibrous PLLA scaffold. DAPI‐positive cells were counted on days 2 and 5 after cultivation. The mean number of cells in each microscopic field was significantly (p