Evaluation of Brix refractometer as an on-farm tool for colostrum IgG evaluation in Italian beef and dairy cattle

In this study it is hypothesized that there are differences between immunoglobulin G (IgG) content in colostrum from beef (Chianina, Podolica) and dairy (Holstein Friesian) cows and that variables such as breed, and parity can influence IgG content. The further objective was to determine if these fa...

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Bibliographic Details
Published in:Journal of dairy research 2021-05, Vol.88 (2), p.189-193
Main Authors: Pisello, Lorenzo, Forte, Claudio, D'Avino, Nicoletta, Pisano, Rocco, Hyatt, Doreene Rose, Rueca, Fabrizio, Passamonti, Fabrizio
Format: Article
Language:English
Subjects:
Beef
Beef cattle
Colostrum
Dairy cattle
Dairy farming
Error reduction
IgG antibody
Immunodiffusion
Immunoglobulin G
Immunoglobulins
Immunology
Milking
Parity
Parturition
Sensitivity
Software
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Summary:In this study it is hypothesized that there are differences between immunoglobulin G (IgG) content in colostrum from beef (Chianina, Podolica) and dairy (Holstein Friesian) cows and that variables such as breed, and parity can influence IgG content. The further objective was to determine if these factors may vary in terms of sensitivity, specificity and the cut point when data obtained with the digital Brix refractometer is compared with the gold standard radial immunodiffusion assay (RID). A total of 90 samples of first-milking colostrum were collected within 2 h after parturition. IgG concentration was determined indirectly by digital Brix refractometer and directly by RID. Results obtained by RID were compared among breed and parity. For the digital Brix refractometer, sensitivity and specificity to detect colostrum with an IgG concentration lower than 50 g/l were calculated and the optimal cut-point was selected for each breed. Samples containing less than
ISSN:0022-0299
1469-7629
DOI:10.1017/S0022029921000315