Extraction of chitin from Litopenaeus vannamei shell and its subsequent characterization: an approach of waste valorization through microbial bioprocessing

Chemical extraction of chitin is very hazardous and costly which can be overwhelmed by microbial bioprocessing. In this study, potent protease and lactic acid-producing bacteria were screened and identified as Alcaligens faecalis S3 and Bacillus coagulans L2, respectively. Productions of protease an...

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Bibliographic Details
Published in:Bioprocess and biosystems engineering 2021-09, Vol.44 (9), p.1943-1956
Main Authors: Rakshit, Subham, Mondal, Subhadeep, Pal, Kalyanbrata, Jana, Arijit, Soren, Jyoti Prakash, Barman, Prasenjit, Mondal, Keshab Chandra, Halder, Suman Kumar
Format: Article
Language:English
Subjects:
Alcaligenes faecalis - growth & development
Animals
Bacillus coagulans - growth & development
Bioprocessing
Biotechnology
Chemical extraction
Chemistry
Chemistry and Materials Science
Chitin
Chitin - chemistry
Chitin - metabolism
Crustaceans
Environmental Engineering/Biotechnology
Food Science
Industrial and Production Engineering
Industrial Chemistry/Chemical Engineering
Ions
Lactic acid
Litopenaeus vannamei
Microorganisms
NMR
Nuclear magnetic resonance
Penaeidae - chemistry
Protease
Proteinase
Research Paper
Thermodynamic properties
Waste Management
X ray photoelectron spectroscopy
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Summary:Chemical extraction of chitin is very hazardous and costly which can be overwhelmed by microbial bioprocessing. In this study, potent protease and lactic acid-producing bacteria were screened and identified as Alcaligens faecalis S3 and Bacillus coagulans L2, respectively. Productions of protease and lactic acid by the respective bacterial strains were optimized. The shell of Litopenaeus vannamei was sequentially treated with the partially purified protease and lactic acid and the treatment conditions were optimized for betterment of chitin yield. Spectral characterization by SEM–EDS, IR, XRD, NMR, XPS and thermal characterization by TG and DTG analysis of the extracted chitin was made and compared with commercial one. It was revealed that both the chitin have similar characteristics. Therefore, it can be articulated that chitin can be extracted from crustacean shells in pure form by microbial bioprocessing which will be a good catch for biorefinary industries for chitin extraction through greener route. Graphic abstract
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-021-02574-y