Occurrence of common plastic additives and contaminants in mussel samples: Validation of analytical method based on matrix solid-phase dispersion

• Published in: Food chemistry 2021-07, Vol.349, p.129169, Article 129169
• Main Authors: Cañadas, R., Garrido Gamarro, E., Garcinuño Martínez, R.M., Paniagua González, G., Fernández Hernando, P.
• Format: Article
• Language: English
• Subjects: accuracy; Alkylphenols; Animals; bisphenol A; Bisphenols; Bivalvia - chemistry; calibration; Chromatography, High Pressure Liquid - methods; DDD (pesticide); DDE (pesticide); DDT (pesticide); dibutyl phthalate; diethyl phthalate; Endocrine Disruptors - analysis; food chemistry; High-performance liquid chromatography; Limit of Detection; Matrix solid-phase dispersion; Mussel samples; mussels; nonylphenols; occurrence; Phthalates; Plastic additives; plastics; Plastics - analysis; quality control; repeatability; solid phase extraction; Solid Phase Extraction - methods; solvents; sorbents; Spain; Water Pollutants, Chemical - analysis
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2021.129169

•A novel approach for endocrine-disrupting chemicals determination in mussels.•Development of a matrix solid phase dispersion methodology for mussel samples.•Simultaneous separation and quantification of 11 EDCs by HPLC-DAD.•Limits of quantification ranged between 0.25 and 16.20 µg/Kg. A new matrix solid-phase dispersion (MSPD) extraction methodology, combined with high-performance liquid chromatography equipped with a diode-array detector, was developed and validated for the simultaneous determination of 10 compounds in mussels from Galician Rias (Spain). These pollutants are compounds commonly used for plastic production as additives, as well as common plastic contaminants. The compounds selected were bisphenol-A, bisphenol-F, bisphenol-S, nonylphenol-9, nonylphenol, diethyl phthalate, dibutyl phthalate, di-2-ethylhexyl phthalate, dichlorodiphenyltrichloroethane, dichlorodiphenyldichloroethane, and dichlorodiphenyldichloroethylene. The parameters affecting the MSPD extraction efficiency such as the type of sorbent, mass sample-sorbent ratio, and extraction solvent were optimised. The proposed method provided satisfactory quantitative recoveries (80–100%), with relative standard deviations lower than 7%. In all cases, the matrix-matched calibration curves were linear in the concentration range of 0.32–120.00 µg/kg, with quantification limits of 0.25–16.20 µg/kg. The novel developed MSPD-high-performance liquid chromatography methodology provided good sensitivity, accuracy, and repeatability for quality control analysis in mussels.