Effects of inhibitions by sodium ion and ammonia and different inocula on acetate-utilizing methanogenesis: Methanogenic activity and succession of methanogens

•Initial methanogen community structures affected the degree of inhibition.•The dominance with M.concilii underwent higher inhibition, but M. bourgensis less.•The succession of methanogens was determined by the conditions of Na+ and NH3.•M.concilii became dominant in the non-inhibitory condition.•M....

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Bibliographic Details
Published in:Bioresource technology 2021-08, Vol.334, p.125202-125202, Article 125202
Main Authors: Lee, Joonyeob, Kim, Eunji, Hwang, Seokhwan
Format: Article
Language:English
Subjects:
Ammonia
Anaerobic digestion
Inhibition
Methanogens
Sodium ion
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Summary:•Initial methanogen community structures affected the degree of inhibition.•The dominance with M.concilii underwent higher inhibition, but M. bourgensis less.•The succession of methanogens was determined by the conditions of Na+ and NH3.•M.concilii became dominant in the non-inhibitory condition.•M. bourgensis became dominant under the combined inhibition by Na+ and NH3. Acetate-fed anaerobic sequential batch experiments with four different inhibitory conditions (non-inhibitory (Lo), sodium-ion inhibitory (Na), ammonia inhibitory (Am), combined inhibitory (Hi)) were conducted using thirteen different inocula to investigate the inhibition effects by sodium-ion and ammonia and different inocula on acetate-utilizing methanogenesis and succession of methanogens. Sodium-ion and ammonia significantly extended lag-time λ and reduced specific-methanogenic-activity RCH4, and caused synergistic inhibition. The inhibition differed according to the initial methanogen community structures: the inhibition effects on λ and RCH4 were strongest ininocula with Methanosaeta concilii dominant and weakest in inocula with Methanoculleus bourgensis dominant. These inhibitory conditions determined the succession of methanogens: the most competitive methanogens were Methanosaeta concilii in Lo, Methanosarcina sp. in Na, Methanosarcina sp. and Methanoculleus bourgensis in Am, Methanoculleus bourgensis in Hi. This study provides valuable information for microbial management and optimization for AD processes treating wastewater that is rich in protein and/or salt.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2021.125202