Redox resetting of cisplatin-resistant ovarian cancer cells by cisplatin-encapsulated nanostructured lipid carriers

To sensitize cisplatin (Cis)-resistant ovarian cancer cells toward Cis using Cis-loaded nanostructured lipid carriers (CisNLCs). CisNLCs were synthesized and characterized using dynamic light scattering, Fourier transform IR and x-ray diffraction (XRD). Sensitivity of PA-1 and CaOV3 cells to Cis and...

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Bibliographic Details
Published in:Nanomedicine (London, England) England), 2021-05, Vol.16 (12), p.979-995
Main Authors: Mittal, Disha, Biswas, Largee, Verma, Anita Kamra
Format: Article
Language:English
Subjects:
ATP7B
cisplatin
GSH
nanostructured lipid carriers
ovarian cancer
reactive oxygen species
redox resetting
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Summary:To sensitize cisplatin (Cis)-resistant ovarian cancer cells toward Cis using Cis-loaded nanostructured lipid carriers (CisNLCs). CisNLCs were synthesized and characterized using dynamic light scattering, Fourier transform IR and x-ray diffraction (XRD). Sensitivity of PA-1 and CaOV3 cells to Cis and its biotoxicity were assessed. Further, expression of the Cis-resistance markers and  , and apoptotic markers , and were quantified by real-time PCR. The size of synthesized CisNLCs was approximately 179.3 ± 2.32 nm and surface charge was -33.9 ± 1.47 mV. IC was 210 μg/ml in PA-1 and 500 μg/ml in CaOV3. CisNLCs modulated reactive oxygen species levels in CaOV3 cells. Reduced and decreased Cis efflux via sequestration caused Cis to accumulate in cytoplasm, thereby augmenting apoptosis in cells. CisNLCs sensitize CaOV3 by redox resetting, indicating their immense therapeutic potential.
ISSN:1743-5889
1748-6963
DOI:10.2217/nnm-2020-0400