Special Focus Issue Part II: Recruitment of solid lipid nanoparticles for the delivery of CRISPR/Cas9: primary evaluation of anticancer gene editing

The CRISPR/Cas9 system is a promising gene-editing tool for various anticancer therapies; however, development of a biocompatible, nonviral and efficient delivery of CRISPR/Cas9 expression systems remains a challenge. Solid lipid nanoparticles (SLNs) were produced based on pseudo and 3D ternary plot...

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Bibliographic Details
Published in:Nanomedicine (London, England) England), 2021-05, Vol.16 (12), p.963-978
Main Authors: Akbaba, Hasan, Erel-Akbaba, Gulsah, Senturk, Serif
Format: Article
Language:English
Subjects:
3D ternary plot
CRISPR/Cas9
gene delivery
PX458
solid lipid nanoparticle
transfection
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Summary:The CRISPR/Cas9 system is a promising gene-editing tool for various anticancer therapies; however, development of a biocompatible, nonviral and efficient delivery of CRISPR/Cas9 expression systems remains a challenge. Solid lipid nanoparticles (SLNs) were produced based on pseudo and 3D ternary plots. Obtained SLNs and their complexes with PX458 plasmid DNA were characterized and evaluated in terms of cytotoxicity and transfection efficiency. SLNs were found to be nanosized, monodispersed, stable and nontoxic. Furthermore, they revealed similar transfection efficiency as the positive control. Overall, we have achieved a good SLN basis for CRISPR/Cas9 delivery and have the potential to produce SLNs with targeted anticancer properties by modifying production parameters and components to facilitate translating CRISPR/Cas9 into preclinical studies.
ISSN:1743-5889
1748-6963
DOI:10.2217/nnm-2020-0412