The role of Mig1, Mig2, Tup1 and Hap4 transcription factors in regulation of xylose and glucose fermentation in the thermotolerant yeast Ogataea polymorpha

ABSTRACT Glucose is a preferred carbon source for most living organisms. The metabolism and regulation of glucose utilization are well studied mostly for Saccharomyces cerevisiae. Xylose is the main pentose sugar released from the lignocellulosic biomass, which has a high potential as a renewable fe...

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Published in:FEMS yeast research 2021-06, Vol.21 (4), p.1
Main Authors: Kurylenko, Olena, Ruchala, Justyna, Kruk, Barbara, Vasylyshyn, Roksolana, Szczepaniak, Justyna, Dmytruk, Kostyantyn, Sibirny, Andriy
Format: Article
Language:English
Subjects:
Biofuels
Carbon sources
Dextrose
DNA binding proteins
Ethanol
Fermentation
Gene regulation
Glucose
Glucose metabolism
Hap4 protein
Metabolism
Physiological aspects
Saccharomyces cerevisiae
Transcription factors
Xylose
Yeast
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Summary:ABSTRACT Glucose is a preferred carbon source for most living organisms. The metabolism and regulation of glucose utilization are well studied mostly for Saccharomyces cerevisiae. Xylose is the main pentose sugar released from the lignocellulosic biomass, which has a high potential as a renewable feedstock for bioethanol production. The thermotolerant yeast Ogataea (Hansenula) polymorpha, in contrast to S. cerevisiae, is able to metabolize and ferment not only glucose but also xylose. However, in non-conventional yeasts, the regulation of glucose and xylose metabolism remains poorly understood. In this study, we characterize the role of transcriptional factors Mig1, Mig2, Tup1 and Hap4 in the natural xylose-fermenting yeast O. polymorpha. The deletion of MIG1 had no significant influence on ethanol production either from xylose or glucose, however the deletion of both MIG1 and MIG2 reduced the amount of ethanol produced from these sugars. The deletion of HAP4-A and TUP1 genes resulted in increased ethanol production from xylose. Inversely, the overexpression of HAP4-A and TUP1 genes reduced ethanol production during xylose alcoholic fermentation. Thus, HAP4-A and TUP1 are involved in repression of xylose metabolism and fermentation in yeast O. polymorpha and their deletion could be a viable strategy to improve ethanol production from this pentose. The investigation of the role of transcriptional factors in xylose and glucose metabolism and fermentation in thermotolerant yeast Ogataea polymorpha.
ISSN:1567-1364
1567-1356
1567-1364
DOI:10.1093/femsyr/foab029