Succinic anhydride-based chemical modification making laccase@Cu3(PO4)2 hybrid nanoflowers robust in removing bisphenol A in wastewater

To prepare a robust biocatalyst and enhance the removal of bisphenol A in wastewater, succinic anhydride was reacted with laccase to obtain succinic anhydride-modified laccase (SA-laccase) and then co-crystallized with Cu 3 (PO 4 ) 2 to form SA-laccase@Cu 3 (PO 4 ) 2 hybrid nanoflowers (hNFs). The a...

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Published in:Bioprocess and biosystems engineering 2021-10, Vol.44 (10), p.2061-2073
Main Authors: Yang, Huafang, He, Peipei, Yin, Youcheng, Mao, Zhili, Zhang, Jing, Zhong, Changle, Xie, Tian, Wang, Anming
Format: Article
Language:English
Subjects:
Anhydrides
Biocatalysts
Biotechnology
Bisphenol A
Calcium phosphates
Catalytic activity
Chemical modification
Chemistry
Chemistry and Materials Science
Crystallization
Environmental Engineering/Biotechnology
Epoxy resins
Food Science
High temperature
Industrial and Production Engineering
Industrial Chemistry/Chemical Engineering
Laccase
Persistent organic pollutants
pH effects
Pollutants
Research Paper
Robustness
Temperature tolerance
Tricalcium phosphate
Wastewater
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Summary:To prepare a robust biocatalyst and enhance the removal of bisphenol A in wastewater, succinic anhydride was reacted with laccase to obtain succinic anhydride-modified laccase (SA-laccase) and then co-crystallized with Cu 3 (PO 4 ) 2 to form SA-laccase@Cu 3 (PO 4 ) 2 hybrid nanoflowers (hNFs). The activity of SA-laccase@Cu 3 (PO 4 ) 2 reached 5.27 U/mg, 1.86-, 2.88- and 2.15-fold those of bare laccase@Cu 3 (PO 4 ) 2 , laccase@Ca 3 (PO 4 ) 2 and laccase@epoxy resin, respectively. Compared with free laccase, the obtained hNFs present enhanced activity and tolerance to pH and high temperature in the removal of BPA. Under the optimum conditions of pH 6.0 and 35 °C, BPA removal reached 93.2% using SA-laccase@Cu 3 (PO 4 ) 2 hNFs, which was 1.21-fold of that using free laccase. In addition, the obtained SA-laccase@Cu 3 (PO 4 ) 2 hNFs retained nearly 90% of their initial catalytic activity for BPA removal after 8 consecutive batch cycles. This efficient method for preparing immobilized laccase can also be further developed and improved to acquire green biocatalysts for removing persistent organic pollutants in wastewater. Graphic abstract
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-021-02583-x