Establishment of lncRNA-mRNA network in bovine oocyte between germinal vesicle and metaphase II stage

•The core lncRNA-mRNA network during the transition of bovine oocyte from GV to MII was identified.•MSTRG.19140-MAD1L1, MSTRG.22465-PRKACB and MSTRG.18119-SMAD4 lncRNA-mRNA networks may affect oocyte maturation.•MSTRG.19140-MAD1L1 was likely involved in the regulation of three signaling pathways inc...

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Bibliographic Details
Published in:Gene 2021-07, Vol.791, p.145716-145716, Article 145716
Main Authors: Li, Ming-Hao, Niu, Meng-Han, Feng, Yan-Qin, Zhang, Shu-Er, Tang, Shao-Wei, Wang, Jun-Jie, Cao, Hong-Guo, Shen, Wei
Format: Article
Language:English
Subjects:
Cow
LncRNA-mRNA network
Meiosis
Oocytes
RNA-seq
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Summary:•The core lncRNA-mRNA network during the transition of bovine oocyte from GV to MII was identified.•MSTRG.19140-MAD1L1, MSTRG.22465-PRKACB and MSTRG.18119-SMAD4 lncRNA-mRNA networks may affect oocyte maturation.•MSTRG.19140-MAD1L1 was likely involved in the regulation of three signaling pathways including bovine oocyte meiosis. Long non-coding RNA (lncRNA), a type of non-protein coding transcripts with lengths exceeding 200 nucleotides, is reported to be widely involved in many cellular and developmental processes. However, few roles of lncRNA in oocyte development have been defined. In this study, to uncover the effect of lncRNA during oocyte maturation, bovine germinal vesicle (GV) and in vitro matured metaphase II (MII) oocytes underwent RNA sequencing. Results revealed a wealth of candidate lncRNAs, which might participate in the biological processes of stage-specific oocytes. Furthermore, their trans- and cis-regulatory effects were investigated in-depth by using bioinformatic software. Functional enrichment analysis of target genes showed that these lncRNAs were likely involved in the regulation of many key signaling pathways during bovine oocyte maturation from GV to MII stage, as well as multiple lncRNA-mRNA networks. One novel lncRNA (MSTRG.19140) was particularly interesting, as it appeared to mediate the regulation of oocyte meiotic resumption, progesterone-mediated oocyte maturation, and cell cycle. Therefore, this study enhanced insights into the regulation of molecular mechanisms of bovine oocyte maturation from a lncRNA-mRNA network perspective.
ISSN:0378-1119
1879-0038
DOI:10.1016/j.gene.2021.145716