Flavobacterium inviolabile sp. nov. isolated from stream water

A Gram-stain-negative, rod-shaped, aerobic and non-motile bacterium, designated P2-65 T , was isolated from Moonsan stream water in the Republic of Korea. The temperature, NaCl concentration and pH ranges for growth of strain P2-65 T were 10–37 °C, 0.0–3.0% (w/v) and 6.5–8.5 with optimum growth at 2...

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Bibliographic Details
Published in:Archives of microbiology 2021-08, Vol.203 (6), p.3633-3639
Main Authors: Van Le, Ve, Lee, Haeseong, Padakandla, Shalem Raj, Cha, In-Tae, Lee, Ki-Eun, Chae, Jong-Chan
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biotechnology
Cell Biology
Deoxyribonucleic acid
DNA
Ecology
Fatty acids
Flavobacterium
Gram stain
Hybridization
Life Sciences
Lipids
Menaquinones
Microbial Ecology
Microbiology
New species
Nucleotides
Original Paper
Phosphatidylethanolamine
Quinones
rRNA 16S
Sodium chloride
Stream water
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Summary:A Gram-stain-negative, rod-shaped, aerobic and non-motile bacterium, designated P2-65 T , was isolated from Moonsan stream water in the Republic of Korea. The temperature, NaCl concentration and pH ranges for growth of strain P2-65 T were 10–37 °C, 0.0–3.0% (w/v) and 6.5–8.5 with optimum growth at 25–30 °C, 0.0–1.0% and 7.0–7.5, respectively. Comparison of 16S rRNA gene sequence showed that strain P2-65 T was closely related to Flavobacterium cauense (95.4%) and Flavobacterium cheniae (95.3%). The major fatty acids were iso-C 15:0 , iso C 17:0 3-OH, summed feature 3 (C 16:1 ω7c and/or C 16:1 ω6c ), summed feature 9 (iso-C 17:1 ω 9c and/or 10-methyl C 16:0 ) and iso-C 15:0 3-OH. The predominant respiratory quinone was menaquinone-6 (MK-6). The major polar lipids detected in the strain were phosphatidylethanolamine, one aminophospholipid, one unidentified aminolipid and one unidentified polar lipid. The G + C content of the genomic DNA was 39.7%. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values for strain P2-65 T with closely related Flavobacterium species were below 74.8% and 20%, respectively. Based on polyphasic features, strain P2-65 T is considered to represent a novel species of the genus Flavobacterium , for which the name Flavobacterium inviolabile sp. nov. is proposed. The type strain is P2-65 T (= KCTC 62055 T  = NBRC 112953 T ).
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-021-02221-w