Prokaryotic diversity and biogeochemical characteristics of field living and laboratory cultured stromatolites from the hypersaline Laguna Interna, Salar de Atacama (Chile)

• Published in: Extremophiles : life under extreme conditions 2021-07, Vol.25 (4), p.327-342
• Main Authors: Osman, Jorge R., Viedma, Pabla, Mendoza, Jorge, Fernandes, Gustavo, DuBow, Michael S., Cotoras, Davor
• Format: Article
• Language: English
• Subjects: Aquaria; Aquariums; Biochemistry; Biogeochemistry; Biomedical and Life Sciences; Biotechnology; Carbon fixation; Community composition; Deoxyribonucleic acid; DNA; Genes; Lakes; Life Sciences; Metabolism; Microbial activity; Microbial Ecology; Microbiology; Microorganisms; Minerals; Nucleotide sequence; Original Paper; PCR; rRNA 16S; Saline environments; Salt lakes; Sediment; Sedimentary structures; Sediments; Space life sciences; Stromatolites; Sulfur; Sulphur; X-ray diffraction
• ISSN: 1431-0651; 1433-4909
• DOI: 10.1007/s00792-021-01232-1

Stromatolites are organo-sedimentary structures found principally in seas and saline lakes that contain sheets of sediments and minerals formed by layers of microbial communities, which trap sediments and induce the precipitation of minerals. A living stromatolite from the alkaline Laguna Interna in the Salar de Atacama was collected and one of the fragments was deposited in an experimental aquarium for 18 months. We used Illumina sequencing of PCR-amplified V4 regions of 16S rRNA genes from total extracted DNA to identify the microbial populations. The chemical structure was studied using X-Ray Diffraction (XRD) and bench chemical methods. We found that members belonging to the Proteobacteria , Planctomycetes , Chloroflex i and Bacteroidetes phyla dominated the bacterial communities of the living and aquarium cultured samples. The potential metabolic functionality of the prokaryotic community reveals that sulfur, nitrogen, methane and carbon fixation metabolism functions are present in the samples. This study is the first to provide new insights into the prokaryotic community composition from this unusual aquatic desert site. Further studies will be helpful to obtain a better understanding of the biotic and abiotic mechanisms residing in stromatolites from Laguna Interna, as well as to have better knowledge about the formation of these biosignatures.