Isolation and Genomic Characterization of Echovirus 11 from faeces of a Non-Human Primate in Nigeria

We recently investigated the presence of enteroviruses (EVs) in non-human primates (NHPs) in Northern Nigeria and documented the presence of EV-A76 of South-East Asian ancestry in an NHP. In this study, we go further to ask if we could also find EVs in NHPs indigenous to the forested South-south Nig...

Full description

Saved in:
Bibliographic Details
Published in:EcoHealth 2020-12, Vol.17 (4), p.461-468
Main Authors: Faleye, T. O. C., George, U. E., Klapsa, D., Majumdar, M., Oragwa, A. O., Adewumi, M. O., Martin, J., Adeniji, J. A.
Format: Article
Language:English
Subjects:
5' Untranslated Regions
Animal Ecology
Animals
Assaying
Cell lines
Clinical isolates
Echovirus
Ecosystems
Enterovirus B, Human
Enteroviruses
Environmental Health
Feces
Gene sequencing
Genomes
Genomics
Medicine
Medicine & Public Health
Microbiology
Nigeria
Original Contribution
Phylogeny
Primates
Public Health
Transcription
Water and Health
Whole genome sequencing
Zoonoses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We recently investigated the presence of enteroviruses (EVs) in non-human primates (NHPs) in Northern Nigeria and documented the presence of EV-A76 of South-East Asian ancestry in an NHP. In this study, we go further to ask if we could also find EVs in NHPs indigenous to the forested South-south Nigeria. Fresh faecal samples were collected from the floor of 10 cages housing NHPs in Cross River Nigeria, re-suspended in PBS and subjected to RNA extraction, cDNA synthesis, PanEnt 5′-UTR and PanEnt VP1 PCR assays. None of the samples was positive for the PanEnt VP1 assay, but one sample was positive for PanEnt 5′-UTR PCR. This sample was subsequently inoculated into RD cell line, produced CPE and the isolate analysed by PCR assays, next-generation whole genome sequencing and passage in four different cell lines showing replication in two of them. Analysis of the complete genome of the isolate identified it as an Echovirus 11 (E11) and revealed a recombinant genomic structure. Phylogenetic analysis showed that the E11 NHP strain was related to human clinical isolates suggesting a zoonotic behaviour. We describe the first isolation and complete genome characterization of an E11 obtained from an NHP in Nigeria having zoonotic potential.
ISSN:1612-9202
1612-9210
DOI:10.1007/s10393-021-01515-1