Variation of the Prokaryotic and Eukaryotic Communities After Distinct Methods of Thermal Pretreatment of the Inoculum in Hydrogen-Production Reactors from Sugarcane Vinasse

The aim of this study is to evaluate the effect of different thermal pretreatments of the inoculum on the diversity of the microbial community producing hydrogen from sugarcane vinasse. High-throughput sequencing of the 16S and 18S rRNA genes was performed. The reactor samples were also selected for...

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Bibliographic Details
Published in:Current microbiology 2021-07, Vol.78 (7), p.2682-2694
Main Authors: Magrini, Flaviane Eva, de Almeida, Gabriela Machado, da Maia Soares, Denis, dos Anjos Borges, Luiz Gustavo, Marconatto, Leticia, Giongo, Adriana, Paesi, Suelen
Format: Article
Language:English
Subjects:
Anaerobes
Biomedical and Life Sciences
Bioreactors
Biotechnology
Biotransformation
Clostridium
Eukaryotes
Hydrogen
Hydrogen production
Inoculum
Life Sciences
Microbiology
Microorganisms
Next-generation sequencing
pH effects
Pretreatment
Prokaryotes
Reactors
rRNA 16S
rRNA 18S
Sugarcane
Vinasse
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Summary:The aim of this study is to evaluate the effect of different thermal pretreatments of the inoculum on the diversity of the microbial community producing hydrogen from sugarcane vinasse. High-throughput sequencing of the 16S and 18S rRNA genes was performed. The reactor samples were also selected for the isolation of strict anaerobes. Decreased microbial diversity was observed with increasing pretreatment temperatures, with Firmicutes predominating: 90% to 97%. The highest abundance of Staphylococcus (7.9%) was found in pretreatment at 120 °C / 20 min at pH 6. The fungal analysis revealed a high prevalence of Candida (47%), Agaricomycetes, Pezizomycotina and Aspergillus in assays with higher H 2 production (90° C / 10 min at pH 6). Three species of Clostridium were isolated: C. bifermentans , C. saccharoperbutylacetonicum and C. saccharobutylicum . The isolates were tested separately and in co-cultures for the production of hydrogen. Hydrogen-producing capacity by co-culture of Clostridium species was increased by 18%. Knowing microorganisms and understanding the interaction between eukaryotes and prokaryotes is essential to obtain strategies for biotransformation of vinasse for the production of bioenergy.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-021-02527-4