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Variation of the Prokaryotic and Eukaryotic Communities After Distinct Methods of Thermal Pretreatment of the Inoculum in Hydrogen-Production Reactors from Sugarcane Vinasse
The aim of this study is to evaluate the effect of different thermal pretreatments of the inoculum on the diversity of the microbial community producing hydrogen from sugarcane vinasse. High-throughput sequencing of the 16S and 18S rRNA genes was performed. The reactor samples were also selected for...
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| Published in: | Current microbiology 2021-07, Vol.78 (7), p.2682-2694 |
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| container_title | Current microbiology |
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| creator | Magrini, Flaviane Eva de Almeida, Gabriela Machado da Maia Soares, Denis dos Anjos Borges, Luiz Gustavo Marconatto, Leticia Giongo, Adriana Paesi, Suelen |
| description | The aim of this study is to evaluate the effect of different thermal pretreatments of the inoculum on the diversity of the microbial community producing hydrogen from sugarcane vinasse. High-throughput sequencing of the 16S and 18S rRNA genes was performed. The reactor samples were also selected for the isolation of strict anaerobes. Decreased microbial diversity was observed with increasing pretreatment temperatures, with Firmicutes predominating: 90% to 97%. The highest abundance of
Staphylococcus
(7.9%) was found in pretreatment at 120 °C / 20 min at pH 6. The fungal analysis revealed a high prevalence of
Candida
(47%), Agaricomycetes, Pezizomycotina and
Aspergillus
in assays with higher H
2
production (90° C / 10 min at pH 6). Three species of Clostridium were isolated:
C. bifermentans
,
C. saccharoperbutylacetonicum
and
C. saccharobutylicum
. The isolates were tested separately and in co-cultures for the production of hydrogen. Hydrogen-producing capacity by co-culture of
Clostridium
species was increased by 18%. Knowing microorganisms and understanding the interaction between eukaryotes and prokaryotes is essential to obtain strategies for biotransformation of vinasse for the production of bioenergy. |
| doi_str_mv | 10.1007/s00284-021-02527-4 |
| format | article |
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Staphylococcus
(7.9%) was found in pretreatment at 120 °C / 20 min at pH 6. The fungal analysis revealed a high prevalence of
Candida
(47%), Agaricomycetes, Pezizomycotina and
Aspergillus
in assays with higher H
2
production (90° C / 10 min at pH 6). Three species of Clostridium were isolated:
C. bifermentans
,
C. saccharoperbutylacetonicum
and
C. saccharobutylicum
. The isolates were tested separately and in co-cultures for the production of hydrogen. Hydrogen-producing capacity by co-culture of
Clostridium
species was increased by 18%. Knowing microorganisms and understanding the interaction between eukaryotes and prokaryotes is essential to obtain strategies for biotransformation of vinasse for the production of bioenergy.</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s00284-021-02527-4</identifier><identifier>PMID: 34013423</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Anaerobes ; Biomedical and Life Sciences ; Bioreactors ; Biotechnology ; Biotransformation ; Clostridium ; Eukaryotes ; Hydrogen ; Hydrogen production ; Inoculum ; Life Sciences ; Microbiology ; Microorganisms ; Next-generation sequencing ; pH effects ; Pretreatment ; Prokaryotes ; Reactors ; rRNA 16S ; rRNA 18S ; Sugarcane ; Vinasse</subject><ispartof>Current microbiology, 2021-07, Vol.78 (7), p.2682-2694</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-e30ac4be62c4a935e7f22d2aa332cd57f4a2c04ec31754eb47f6cdd7ea3a53cc3</citedby><cites>FETCH-LOGICAL-c375t-e30ac4be62c4a935e7f22d2aa332cd57f4a2c04ec31754eb47f6cdd7ea3a53cc3</cites><orcidid>0000-0001-5763-6649</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34013423$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Magrini, Flaviane Eva</creatorcontrib><creatorcontrib>de Almeida, Gabriela Machado</creatorcontrib><creatorcontrib>da Maia Soares, Denis</creatorcontrib><creatorcontrib>dos Anjos Borges, Luiz Gustavo</creatorcontrib><creatorcontrib>Marconatto, Leticia</creatorcontrib><creatorcontrib>Giongo, Adriana</creatorcontrib><creatorcontrib>Paesi, Suelen</creatorcontrib><title>Variation of the Prokaryotic and Eukaryotic Communities After Distinct Methods of Thermal Pretreatment of the Inoculum in Hydrogen-Production Reactors from Sugarcane Vinasse</title><title>Current microbiology</title><addtitle>Curr Microbiol</addtitle><addtitle>Curr Microbiol</addtitle><description>The aim of this study is to evaluate the effect of different thermal pretreatments of the inoculum on the diversity of the microbial community producing hydrogen from sugarcane vinasse. High-throughput sequencing of the 16S and 18S rRNA genes was performed. The reactor samples were also selected for the isolation of strict anaerobes. Decreased microbial diversity was observed with increasing pretreatment temperatures, with Firmicutes predominating: 90% to 97%. The highest abundance of
Staphylococcus
(7.9%) was found in pretreatment at 120 °C / 20 min at pH 6. The fungal analysis revealed a high prevalence of
Candida
(47%), Agaricomycetes, Pezizomycotina and
Aspergillus
in assays with higher H
2
production (90° C / 10 min at pH 6). Three species of Clostridium were isolated:
C. bifermentans
,
C. saccharoperbutylacetonicum
and
C. saccharobutylicum
. The isolates were tested separately and in co-cultures for the production of hydrogen. Hydrogen-producing capacity by co-culture of
Clostridium
species was increased by 18%. Knowing microorganisms and understanding the interaction between eukaryotes and prokaryotes is essential to obtain strategies for biotransformation of vinasse for the production of bioenergy.</description><subject>Anaerobes</subject><subject>Biomedical and Life Sciences</subject><subject>Bioreactors</subject><subject>Biotechnology</subject><subject>Biotransformation</subject><subject>Clostridium</subject><subject>Eukaryotes</subject><subject>Hydrogen</subject><subject>Hydrogen production</subject><subject>Inoculum</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Next-generation sequencing</subject><subject>pH effects</subject><subject>Pretreatment</subject><subject>Prokaryotes</subject><subject>Reactors</subject><subject>rRNA 16S</subject><subject>rRNA 18S</subject><subject>Sugarcane</subject><subject>Vinasse</subject><issn>0343-8651</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kU1PVDEYhRujkRH9Ay5IEzdurvbzXu6SjCOQYDSKbJtO-96Zwm0L_Vjwo_yPFAYwceGiaZo-73lPzkHoPSWfKCHD50wIOxQdYbQdyYZOvEALKjjryDjSl2hBuODdYS_pHnqT8yUhlI2EvkZ7XBDKBeML9OdCJ6eLiwHHCZct4B8pXul0G4szWAeLV_X5uYze1-CKg4yPpgIJf3G5uGAK_gZlG22-FznfQvJ6bkJQEujiIZQn8dMQTZ2rxy7gk1ub4gZC1zbaah48_ARtSkwZTyl6_KtudDI6AL5wQecMb9GrSc8Z3j3e--j319X58qQ7-358ujw66wwfZOmAE23EGnpmhB65hGFizDKtOWfGymESmhkiwHA6SAFrMUy9sXYAzbXkxvB99HGne53iTYVclHfZwDw3L7Fm1dIexxY0ZQ398A96GWsKzV2jBJOc9LJvFNtRJsWcE0zqOjnfclWUqPsy1a5M1cpUD2Uq0YYOHqXr2oN9HnlqrwF8B-T2FTaQ_u7-j-wdxhauAw</recordid><startdate>20210701</startdate><enddate>20210701</enddate><creator>Magrini, Flaviane Eva</creator><creator>de Almeida, Gabriela Machado</creator><creator>da Maia Soares, Denis</creator><creator>dos Anjos Borges, Luiz 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Suelen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Variation of the Prokaryotic and Eukaryotic Communities After Distinct Methods of Thermal Pretreatment of the Inoculum in Hydrogen-Production Reactors from Sugarcane Vinasse</atitle><jtitle>Current microbiology</jtitle><stitle>Curr Microbiol</stitle><addtitle>Curr Microbiol</addtitle><date>2021-07-01</date><risdate>2021</risdate><volume>78</volume><issue>7</issue><spage>2682</spage><epage>2694</epage><pages>2682-2694</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><abstract>The aim of this study is to evaluate the effect of different thermal pretreatments of the inoculum on the diversity of the microbial community producing hydrogen from sugarcane vinasse. High-throughput sequencing of the 16S and 18S rRNA genes was performed. The reactor samples were also selected for the isolation of strict anaerobes. Decreased microbial diversity was observed with increasing pretreatment temperatures, with Firmicutes predominating: 90% to 97%. The highest abundance of
Staphylococcus
(7.9%) was found in pretreatment at 120 °C / 20 min at pH 6. The fungal analysis revealed a high prevalence of
Candida
(47%), Agaricomycetes, Pezizomycotina and
Aspergillus
in assays with higher H
2
production (90° C / 10 min at pH 6). Three species of Clostridium were isolated:
C. bifermentans
,
C. saccharoperbutylacetonicum
and
C. saccharobutylicum
. The isolates were tested separately and in co-cultures for the production of hydrogen. Hydrogen-producing capacity by co-culture of
Clostridium
species was increased by 18%. Knowing microorganisms and understanding the interaction between eukaryotes and prokaryotes is essential to obtain strategies for biotransformation of vinasse for the production of bioenergy.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>34013423</pmid><doi>10.1007/s00284-021-02527-4</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0001-5763-6649</orcidid></addata></record> |
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| ispartof | Current microbiology, 2021-07, Vol.78 (7), p.2682-2694 |
| issn | 0343-8651 1432-0991 |
| language | eng |
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| source | Springer Link |
| subjects | Anaerobes Biomedical and Life Sciences Bioreactors Biotechnology Biotransformation Clostridium Eukaryotes Hydrogen Hydrogen production Inoculum Life Sciences Microbiology Microorganisms Next-generation sequencing pH effects Pretreatment Prokaryotes Reactors rRNA 16S rRNA 18S Sugarcane Vinasse |
| title | Variation of the Prokaryotic and Eukaryotic Communities After Distinct Methods of Thermal Pretreatment of the Inoculum in Hydrogen-Production Reactors from Sugarcane Vinasse |
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