Effects of 1,25 Dihydroxyvitamin D3 on Human Retinal Pigment Epithelial Cell Lines

Purpose To assess the effects of 1,25 dihydroxyvitamin D 3 (vitamin D 3 ) either alone or under oxidative damage on human retinal pigment epithelium cell lines. Methods The human retinal pigment epithelial cell lines were pretreated with hydrogen peroxide with different concentrations (100–1000 μM)...

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Bibliographic Details
Published in:International ophthalmology 2021-10, Vol.41 (10), p.3333-3340
Main Authors: Ekinci, Cansu, Guler, Eray Metin, Kocyigit, Abdurrahim, Kirik, Furkan, Ozdemir, Hakan
Format: Article
Language:English
Subjects:
Age
Age related diseases
Apoptosis
Calcitriol
Cell lines
Cell viability
Cytotoxicity
Damage
DNA damage
Epithelium
Evaluation
Eye diseases
Genotoxicity
Hydrogen
Hydrogen peroxide
Intracellular
Macular degeneration
Medicine
Medicine & Public Health
Membrane potential
Membranes
Mitochondria
Ophthalmology
Original Paper
Oxidative stress
Reactive oxygen species
Retina
Retinal pigment epithelium
Statistical analysis
Vitamin D
Vitamin D3
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Summary:Purpose To assess the effects of 1,25 dihydroxyvitamin D 3 (vitamin D 3 ) either alone or under oxidative damage on human retinal pigment epithelium cell lines. Methods The human retinal pigment epithelial cell lines were pretreated with hydrogen peroxide with different concentrations (100–1000 μM) and durations (4, 12 and 24 h) to determine the appropriate dose. A group of cells were treated with vitamin D 3 alone, and another group of cells were co-treated with different concentrations of (10–100 nM) vitamin D 3 and hydrogen peroxide. Anti-cytotoxic, anti-apoptotic and anti-genotoxic effects of vitamin D 3 on the hydrogen peroxide treated cell line were evaluated. In addition, mitochondrial membrane potentials of treated cell lines were measured. Results Vitamin D 3 showed statistically significant anti-cytotoxic effects and increased cell viability in all concentrations ( p  
ISSN:0165-5701
1573-2630
DOI:10.1007/s10792-021-01895-x