Characterization of Tempranillo negro (VN21), a high phenolic content grapevine Tempranillo clone, through UHPLC-QqQ-MS/MS polyphenol profiling

•Phenolic composition in grape skin and seeds from Tempranillo negro clone.•Targeted UPLC-QqQ-MS/MS metabolomics of grape and wine from Tempranillo negro clone.•Increased phenol extractability during winemaking of Tempranillo negro clone.•Tempranillo clonal selection increasing wine phenol content....

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Bibliographic Details
Published in:Food chemistry 2021-10, Vol.360, p.130049-130049, Article 130049
Main Authors: Royo, Carolina, Ferradás, Yolanda, Martínez-Zapater, José Miguel, Motilva, Maria-Jose
Format: Article
Language:English
Subjects:
Anthocyanins
Berry phenolic composition
Phenolic compounds
Somatic variation
Stilbenes
Tempranillo
Vitis vinifera
Wine phenolic composition
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Summary:•Phenolic composition in grape skin and seeds from Tempranillo negro clone.•Targeted UPLC-QqQ-MS/MS metabolomics of grape and wine from Tempranillo negro clone.•Increased phenol extractability during winemaking of Tempranillo negro clone.•Tempranillo clonal selection increasing wine phenol content. Grapevine cultivar and clone genotype is an important factor in the phenolic composition of wine. In this study, a new intense dark black berry color variant of Tempranillo, known as Tempranillo negro or VN21, is described. A targeted chromatographic approach based on UHPLC-QqQ-MS/MS was used to study the anthocyanins and non-colored phenols of the grape berry (skin and seeds) and wine. RJ43, one of the most cultivated clones in D.O.Ca. Rioja (Spain), was analyzed for comparison. Results suggest that the unique color of the grape skin in Tempranillo negro could be explained by higher concentrations of peonidin and cyanidin derivatives. This genotype accumulated anthocyanins in the seeds. Those differences in the berry were enhanced in the VN21 wines, which displayed notably higher concentrations of anthocyanins, and significantly increased contents of proanthocyanidins and stilbenes. This study exemplifies the application of phenol chromatographic analyses of spontaneous somatic variants to grapevine clonal selection.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2021.130049